|
| Status |
Public on Aug 24, 2007 |
| Title |
Knockout CS_2_9_KO_1 |
| Sample type |
RNA |
| |
|
| Source name |
SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice
|
| Organism |
Mus musculus |
| Characteristics |
8 weeks old sex age matched littermates, lung typeII cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
|
| Label |
biotinylate
|
| Label protocol |
based on Affymetrix protocols
|
| |
|
| Hybridization protocol |
The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
|
| Scan protocol |
Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
|
| Description |
SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
|
| Data processing |
raw data processed by Affymetrix Microarray Suite 5.0 is provided
|
| |
|
| Submission date |
Jan 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yan Xu |
| E-mail(s) |
yan.xu@cchmc.org
|
| Phone |
513-6368921
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6846 |
Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
