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Sample GSM157806 Query DataSets for GSM157806
Status Public on Aug 24, 2007
Title Knockout CS_2_9_KO_1
Sample type RNA
 
Source name SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice
Organism Mus musculus
Characteristics 8 weeks old sex age matched littermates, lung typeII cells
Extracted molecule total RNA
Extraction protocol Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
Label biotinylate
Label protocol based on Affymetrix protocols
 
Hybridization protocol The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
Scan protocol Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
Description SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
Data processing raw data processed by Affymetrix Microarray Suite 5.0 is provided
 
Submission date Jan 23, 2007
Last update date Aug 28, 2018
Contact name Yan Xu
E-mail(s) yan.xu@cchmc.org
Phone 513-6368921
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1261
Series (1)
GSE6846 Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection flag information
DETECTION P-VALUE Detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1633.6 P 0.000754
AFFX-BioB-M_at 1868 P 0.00034
AFFX-BioB-3_at 1253.7 P 0.000147
AFFX-BioC-5_at 5041.4 P 0.000081
AFFX-BioC-3_at 4526.8 P 0.000044
AFFX-BioDn-5_at 8844.6 P 0.000044
AFFX-BioDn-3_at 15259.9 P 0.000127
AFFX-CreX-5_at 43158.7 P 0.000044
AFFX-CreX-3_at 56112.6 P 0.000044
AFFX-DapX-5_at 28.5 A 0.48511
AFFX-DapX-M_at 79.5 A 0.659339
AFFX-DapX-3_at 144.7 A 0.60308
AFFX-LysX-5_at 21.4 A 0.712274
AFFX-LysX-M_at 63.2 A 0.783476
AFFX-LysX-3_at 125.8 A 0.239063
AFFX-PheX-5_at 14.5 A 0.876428
AFFX-PheX-M_at 44 A 0.737173
AFFX-PheX-3_at 125 A 0.559354
AFFX-ThrX-5_at 86.1 A 0.760937
AFFX-ThrX-M_at 21 A 0.834139

Total number of rows: 45101

Table truncated, full table size 1238 Kbytes.




Supplementary file Size Download File type/resource
GSM157806.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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