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Sample GSM157807 Query DataSets for GSM157807
Status Public on Aug 24, 2007
Title Knockout CS_2_15_KO_2
Sample type RNA
 
Source name SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice
Organism Mus musculus
Characteristics 8 weeks old sex age matched littermates, lung typeII cells
Extracted molecule total RNA
Extraction protocol Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
Label biotinylate
Label protocol based on Affymetrix protocols
 
Hybridization protocol The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
Scan protocol Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
Description SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
Data processing raw data processed by Affymetrix Microarray Suite 5.0 is provided
 
Submission date Jan 23, 2007
Last update date Aug 28, 2018
Contact name Yan Xu
E-mail(s) yan.xu@cchmc.org
Phone 513-6368921
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1261
Series (1)
GSE6846 Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection flag information
DETECTION P-VALUE Detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1905.8 P 0.000754
AFFX-BioB-M_at 2875.9 P 0.00011
AFFX-BioB-3_at 1648.1 P 0.000081
AFFX-BioC-5_at 5838 P 0.000052
AFFX-BioC-3_at 7589.2 P 0.000044
AFFX-BioDn-5_at 11310.5 P 0.000044
AFFX-BioDn-3_at 19945.9 P 0.000081
AFFX-CreX-5_at 63486.4 P 0.000052
AFFX-CreX-3_at 74363.5 P 0.000044
AFFX-DapX-5_at 124.6 M 0.058428
AFFX-DapX-M_at 95.4 A 0.354428
AFFX-DapX-3_at 83 A 0.617401
AFFX-LysX-5_at 39.8 A 0.313693
AFFX-LysX-M_at 19.5 A 0.953518
AFFX-LysX-3_at 17.1 A 0.41138
AFFX-PheX-5_at 7.7 A 0.814953
AFFX-PheX-M_at 10.4 A 0.960352
AFFX-PheX-3_at 105.1 A 0.340648
AFFX-ThrX-5_at 40.6 A 0.724854
AFFX-ThrX-M_at 21.6 A 0.852061

Total number of rows: 45101

Table truncated, full table size 1238 Kbytes.




Supplementary file Size Download File type/resource
GSM157807.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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