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Sample GSM157834 Query DataSets for GSM157834
Status Public on Aug 24, 2007
Title Knockout CS_MA_5_11_KO_3
Sample type RNA
 
Source name SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice
Organism Mus musculus
Characteristics 8 weeks old sex age matched littermates, lung typeII cells
Extracted molecule total RNA
Extraction protocol Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
Label biotinylate
Label protocol based on Affymetrix protocols
 
Hybridization protocol The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
Scan protocol Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
Description SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
Data processing raw data processed by Affymetrix Microarray Suite 5.0 is provided
 
Submission date Jan 23, 2007
Last update date Aug 28, 2018
Contact name Yan Xu
E-mail(s) yan.xu@cchmc.org
Phone 513-6368921
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1261
Series (1)
GSE6846 Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection flag information
DETECTION P-VALUE Detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1729.8 P 0.000972
AFFX-BioB-M_at 2736.8 P 0.000147
AFFX-BioB-3_at 1395.4 P 0.000095
AFFX-BioC-5_at 5896.2 P 0.00006
AFFX-BioC-3_at 6179.1 P 0.000044
AFFX-BioDn-5_at 10243.7 P 0.000052
AFFX-BioDn-3_at 17372.9 P 0.000147
AFFX-CreX-5_at 57911.1 P 0.000052
AFFX-CreX-3_at 74253.9 P 0.000044
AFFX-DapX-5_at 74.9 A 0.262827
AFFX-DapX-M_at 25 A 0.852061
AFFX-DapX-3_at 14.6 A 0.966323
AFFX-LysX-5_at 27.9 A 0.645547
AFFX-LysX-M_at 171.6 A 0.440646
AFFX-LysX-3_at 71.5 A 0.313723
AFFX-PheX-5_at 23.5 A 0.58862
AFFX-PheX-M_at 13.2 A 0.953533
AFFX-PheX-3_at 199.5 A 0.382599
AFFX-ThrX-5_at 102.4 A 0.368438
AFFX-ThrX-M_at 89.5 A 0.425962

Total number of rows: 45101

Table truncated, full table size 1238 Kbytes.




Supplementary file Size Download File type/resource
GSM157834.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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