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Sample GSM157835 Query DataSets for GSM157835
Status Public on Aug 24, 2007
Title Wild-type CS_12_29_1_WT_1
Sample type RNA
 
Source name SP-C-rtTA/(tetO)7CMV-Cre double transgenic mice
Organism Mus musculus
Characteristics 8 weeks old sex age matched littermates, lung typeII cells
Extracted molecule total RNA
Extraction protocol Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
Label biotinylate
Label protocol based on Affymetrix protocols
 
Hybridization protocol The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
Scan protocol Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
Description SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
Data processing raw data processed by Affymetrix Microarray Suite 5.0 is provided
 
Submission date Jan 23, 2007
Last update date Aug 28, 2018
Contact name Yan Xu
E-mail(s) yan.xu@cchmc.org
Phone 513-6368921
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL1261
Series (1)
GSE6846 Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection flag information
DETECTION P-VALUE Detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1559.6 P 0.000972
AFFX-BioB-M_at 2542.7 P 0.000127
AFFX-BioB-3_at 1543.7 P 0.000662
AFFX-BioC-5_at 5486.4 P 0.000052
AFFX-BioC-3_at 7065.3 P 0.000044
AFFX-BioDn-5_at 9299.3 P 0.000052
AFFX-BioDn-3_at 17595.5 P 0.000225
AFFX-CreX-5_at 57112.7 P 0.000052
AFFX-CreX-3_at 72876.2 P 0.000044
AFFX-DapX-5_at 150.3 P 0.039661
AFFX-DapX-M_at 66 A 0.60308
AFFX-DapX-3_at 73.1 A 0.712257
AFFX-LysX-5_at 9.5 A 0.814869
AFFX-LysX-M_at 18.3 A 0.932322
AFFX-LysX-3_at 101.1 A 0.250796
AFFX-PheX-5_at 13.3 A 0.876428
AFFX-PheX-M_at 14 A 0.937071
AFFX-PheX-3_at 177.2 A 0.382599
AFFX-ThrX-5_at 68.6 A 0.659339
AFFX-ThrX-M_at 19.1 A 0.724854

Total number of rows: 45101

Table truncated, full table size 1235 Kbytes.




Supplementary file Size Download File type/resource
GSM157835.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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