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Sample GSM157836 Query DataSets for GSM157836
Status Public on Aug 24, 2007
Title Wild-type CS_12_29_2_WT_2
Sample type RNA
Source name SP-C-rtTA/(tetO)7CMV-Cre double transgenic mice
Organism Mus musculus
Characteristics 8 weeks old sex age matched littermates, lung typeII cells
Extracted molecule total RNA
Extraction protocol Alveolar type II cells were isolated from 8 weeks old sex age matched littermate control and Stat3∆/∆ mice using collagenase and differential plating. Type II cells from 3 mice were pooled to obtain one pellet. Two hours after isolation, type II cells were homogenized with TRIzol reagent (Invitrogen, Carlsbad, CA). RNA concentration was measured by spectrophotometer and normalized for cDNA synthesis.
Label biotinylate
Label protocol based on Affymetrix protocols
Hybridization protocol The cRNA was then hybridized to the murine genome MOE430 (consists of ≈ 45000 gene entries) chips (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol.
Scan protocol Affymetrix Microarray Suite 5.0 was used to scan and quantitate the gene chips under default scan settings.
Description SP-C-rtTA/(tetO)7CMV-Cre/Stat3flx/flx triple-transgenic mice, typeII cell
Data processing raw data processed by Affymetrix Microarray Suite 5.0 is provided
Submission date Jan 23, 2007
Last update date Aug 28, 2018
Contact name Yan Xu
Phone 513-6368921
Organization name Cincinnati Children's Hospital Medical Center
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platform ID GPL1261
Series (1)
GSE6846 Gene Expression and Biological Processes Influenced By Deletion of Stat3 in pulmonary Alveolar Type II Cells
Reanalyzed by GSE119085

Data table header descriptions
VALUE Signal
ABS_CALL Detection flag information
DETECTION P-VALUE Detection p-value

Data table
AFFX-BioB-5_at 1614.1 P 0.000754
AFFX-BioB-M_at 2680.3 P 0.00011
AFFX-BioB-3_at 1574.1 P 0.000857
AFFX-BioC-5_at 6257.9 P 0.000052
AFFX-BioC-3_at 7583.2 P 0.000052
AFFX-BioDn-5_at 10920 P 0.000044
AFFX-BioDn-3_at 20664.6 P 0.000147
AFFX-CreX-5_at 63060.9 P 0.000052
AFFX-CreX-3_at 81662 P 0.000044
AFFX-DapX-5_at 16.4 A 0.686277
AFFX-DapX-M_at 82.3 A 0.5
AFFX-DapX-3_at 9 A 0.973899
AFFX-LysX-5_at 101.3 A 0.287743
AFFX-LysX-M_at 14.2 A 0.957052
AFFX-LysX-3_at 24.2 A 0.544587
AFFX-PheX-5_at 21 A 0.783476
AFFX-PheX-M_at 15.3 A 0.794268
AFFX-PheX-3_at 52.4 A 0.51489
AFFX-ThrX-5_at 81.9 A 0.645547
AFFX-ThrX-M_at 109.3 A 0.41138

Total number of rows: 45101

Table truncated, full table size 1236 Kbytes.

Supplementary file Size Download File type/resource
GSM157836.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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