NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM159279 Query DataSets for GSM159279
Status Public on Aug 03, 2007
Title WT78
Sample type RNA
 
Source name B6, HFD 2wks
Organism Mus musculus
Characteristics B6 wild type
Age: 4-8 wks
Treatment protocol Mice were feed high-fat diet(TD88137) for 2 weeks
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen Column.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Genome Array.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from wt B6 mice liver HFD 2 weeks.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 29, 2007
Last update date Aug 28, 2018
Contact name Yugang Wang
E-mail(s) yugang@uchicago.edu
Phone 773-702-0928
Fax 773-834-8940
Organization name University of Chicago
Department Pathology
Street address 5841 S. Maryland
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
 
Platform ID GPL1261
Series (1)
GSE6903 Expression data from high-fat diet feeded WT and LIGHT Tg mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF probe set
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 223.65
AFFX-BioB-M_at 271.178
AFFX-BioB-3_at 24.96
AFFX-BioC-5_at 302.512
AFFX-BioC-3_at 273.397
AFFX-BioDn-5_at 437.442
AFFX-BioDn-3_at 2607.58
AFFX-CreX-5_at 4758.77
AFFX-CreX-3_at 6124.23
AFFX-DapX-5_at 46.42
AFFX-DapX-M_at 57.8818
AFFX-DapX-3_at 25.52
AFFX-LysX-5_at 19.5714
AFFX-LysX-M_at 70.5526
AFFX-LysX-3_at 30.1
AFFX-PheX-5_at 6.9
AFFX-PheX-M_at 5.36667
AFFX-PheX-3_at 16.5714
AFFX-ThrX-5_at 15.65
AFFX-ThrX-M_at 46.8571

Total number of rows: 45101

Table truncated, full table size 827 Kbytes.




Supplementary file Size Download File type/resource
GSM159279.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap