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Sample GSM159346 Query DataSets for GSM159346
Status Public on Aug 30, 2007
Title Placenta Adra2bKO 2
Sample type RNA
 
Source name placenta (E 10.5)
Organism Mus musculus
Characteristics The placenta (E 10.5) of a congenic adra2B-/- mouse embryo (maternal genotype Adra2b+/-) was rapidly dissected and flash frozen.
Biomaterial provider Institute of Pharmacology, University of Freiburg
Treatment protocol No additional treatment was applied.
Growth protocol none
Extracted molecule total RNA
Extraction protocol Total RNA (5 µg) from Placenta (E10.5) was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Double stranded cDNA was synthesized (GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents, 900493) and biotin labeled cRNA was in vitro transcribed (GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents, 900493). Cleanup of the cDNA and the fragmented cRNA was carried out with the GeneChip Sample Module (Affymetrix,900371). The integrity of the total RNA and of the subsequent preparation steps was validated using the Agilent 2100 bioanalyzer (Waldbronn, Germany).
 
Hybridization protocol 10 µg of fragmented cRNA were hybridized (16h) to the array. Staining and washing was conducted according to the Affymetrix protocol.
Scan protocol The processed array was scanned using a GeneChip Scanner 3000 7G (Affymetrix).
Description The whole procedure was carried out according to the Affymetrix standard protocol (Eukaryotic Sample and Array Processing).
Data processing Data was analyzed using ArrayAssist 5.0 software (Stratagene). Probe levels were calculated using the GCRMA algorithm.
 
Submission date Jan 30, 2007
Last update date Aug 28, 2018
Contact name Ralf Gilsbach
E-mail(s) Ralf.Gilsbach@pharmakol.uni-freiburg.de
Organization name Institute of Pharmacology
Lab Prof. Dr. Lutz Hein
Street address Albertstr. 25
City Freiburg
ZIP/Postal code 79104
Country Germany
 
Platform ID GPL1261
Series (1)
GSE6909 Differential gene expression in placentae (E10.5) from adra2bKO and adra2bWT mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal intensity calculated by GCRMA

Data table
ID_REF VALUE
1415670_at 843.88995
1415671_at 1255.5167
1415672_at 2476.099
1415673_at 268.4217
1415674_a_at 715.47644
1415675_at 571.33215
1415676_a_at 1719.7885
1415677_at 313.40976
1415678_at 402.51636
1415679_at 1627.8055
1415680_at 361.37326
1415681_at 720.7031
1415682_at 132.33662
1415683_at 1748.0654
1415684_at 211.94495
1415685_at 239.90994
1415686_at 669.295
1415687_a_at 9010.45
1415688_at 513.3597
1415689_s_at 44.625988

Total number of rows: 45101

Table truncated, full table size 923 Kbytes.




Supplementary file Size Download File type/resource
GSM159346.CEL.gz 6.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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