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Sample GSM1593851 Query DataSets for GSM1593851
Status Public on Jan 29, 2015
Title Nax knockdown HaCaT control sodium concentration_3
Sample type RNA
 
Source name keratinocyte cell line
Organism Homo sapiens
Characteristics gender: female
tissue: skin epithelium
cell line: HaCaT
genotype/variation: Nax knockdown
treatment: control
time: control
Treatment protocol A treatment with control medium (final150 mM sodium) or medium plus 10% more sodium (final 165 mM sodium) was applied to each well. Cells were harvested at time point 0, 4, and 16 hours post treatment.
Growth protocol Both wild type and Nax knockdown HaCaT cells were seeded in 12-well plates. 70-80% confluent cells were starved with fetal bovine serum (FBS) free Dulbecco's modiļ¬ed Eagle's medium (DMEM) overnight
Extracted molecule total RNA
Extraction protocol The total RNA from wild type and Nax knockdown HaCaT cells
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Hybridization performed at the Interdisciplinary Center for Biotechnology Research Microarray Core, University of Florida. The microarray chips were Affymetrix GeneChipĀ® Human Transcriptome Array 2.0
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description Sample name: HT_Nx_0_3
Data processing The microarray data was processed with Affymetrix Expression Console and Transcriptome Analysis Console (Affymetrix).
RMA expression value derived from Expression Console software; core-exon analysis
 
Submission date Jan 28, 2015
Last update date Jan 29, 2015
Contact name Wei Xu
E-mail(s) wei-xu@northwestern.edu
Phone 312 908 0566
Organization name Northwestern University, Feinberg School of Medicine
Department Surgery
Lab Laboratory for Wound Repair and Regenerative Medicine
Street address 303 E Chicago Ave, Tarry 4-714
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platform ID GPL17586
Series (1)
GSE65366 Gene expression of human keratinocyte (HaCaT) under the stress of high sodium concentration

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
TC01000001.hg.1 5.89056
TC01000002.hg.1 5.05991
TC01000003.hg.1 2.76584
TC01000004.hg.1 3.39157
TC01000005.hg.1 8.83245
TC01000006.hg.1 11.828
TC01000007.hg.1 11.9426
TC01000008.hg.1 8.41212
TC01000009.hg.1 3.09757
TC01000010.hg.1 3.72676
TC01000011.hg.1 6.55797
TC01000012.hg.1 4.46321
TC01000013.hg.1 8.63549
TC01000014.hg.1 3.41986
TC01000015.hg.1 6.79786
TC01000016.hg.1 6.71935
TC01000017.hg.1 5.41804
TC01000018.hg.1 7.24476
TC01000019.hg.1 6.53014
TC01000020.hg.1 6.90939

Total number of rows: 70523

Table truncated, full table size 1655 Kbytes.




Supplementary file Size Download File type/resource
GSM1593851_HT_Nx_0_3.CEL.gz 25.1 Mb (ftp)(http) CEL
GSM1593851_HT_Nx_0_3.rma-gene-full.chp.gz 459.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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