|
| Status |
Public on Feb 03, 2015 |
| Title |
Primary human monocytes from patient with metastatic breast cancer #3 |
| Sample type |
RNA |
| |
|
| Source name |
Primary human monocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary monocyte
disease state: Metastatic breast cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIZOL. Quality control was performed with Agilent 2100 Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
B.3
|
| Data processing |
Data was handled and normalized in BASE (https://casa4.bmc.lu.se/onk/). All data was mean background corrected followed by quantile normalization. Array control probes were removed.
|
| |
|
| Submission date |
Feb 02, 2015 |
| Last update date |
Feb 03, 2015 |
| Contact name |
Kristoffer von Stedingk |
| E-mail(s) |
kristoffer.von_stedingk@med.lu.se
|
| Organization name |
Lund University
|
| Street address |
Scheelevägen 2
|
| City |
Lund |
| ZIP/Postal code |
223 81 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE65517 |
Comparative analysis of monocytes from healthy donors, patients with metastatic breast cancer, sepsis or tuberculosis. |
|
