|
| Status |
Public on Aug 01, 2007 |
| Title |
empty vector |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells gene-transduced with empty vector
|
| Organism |
Mus musculus |
| Characteristics |
T cells were obtained from BALB/c mice and purified into CD4+ naive T cells (GITRlowCD25-CD4+) and retrovirally gene transduced.
|
| Treatment protocol |
CD4+ naive T cells were activated and retrovirally gene transduced with either empty vector (pMCsIg) or AML1-, wild type Foxp3-, or an AML1-non binding mutant Foxp3. Sixty hours after transfection, GFP+ cells were sorted and lysed for RNA samples.
|
| Growth protocol |
Naive CD4+ T cells were activated by soluble anti-CD3 Ab [0.5ug/ml], IL-2 [20u/ml] and antigen presenting cells (T cell-depleted, mitomycin-C-treated splenocytes). Twenty-four hours after transfection, retroviral supernatant and polybrene were added and centrifuged for one hour at 3200 rpm. Cells were kept at 37 C, 5% CO2 until they were sorted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We performed RNA extraction using QIAGEN's RNeasy micro columns. according to the manufacteur's protocol.
|
| Label |
biotin
|
| Label protocol |
One cycle target labelling according to the protocol provided by Affymetrix
|
| |
|
| Hybridization protocol |
Labelled cRNA (15ug) were hybridized with Mouse Genome 430 2.0 Array for 16 hours, washed, and stained by streptavidin-phycoerthrin.
|
| Scan protocol |
The arrays were scanned and aquired data were analyzed by Gene chip operating software (GCOS).
|
| Description |
Refer to Ono, M. et al, Nature 2007 (Supplementary Figure S7).
|
| Data processing |
Gene chip operating software (GCOS).
|
| |
|
| Submission date |
Feb 02, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Masahiro Ono |
| E-mail(s) |
m.ono@ucl.ac.uk
|
| Organization name |
Institute of Child Health
|
| Department |
Immunobiology Unit
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6939 |
CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1 |
|
| Relations |
| Reanalyzed by |
GSE119085 |
