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Sample GSM160042 Query DataSets for GSM160042
Status Public on Aug 01, 2007
Title Foxp3 mutant
Sample type RNA
 
Source name CD4+ T cells gene-transduced with a Foxp3 mutant defective in binding to AML1.
Organism Mus musculus
Characteristics T cells were obtained from BALB/c mice and puridied to CD4+ naive T cells (GITRlowCD25-CD4+) and retrovirally gene transduced
Treatment protocol CD4+ naive T cells were activated and retrovirally gene transducedwith either empty vector (pMCsIg) or AML1-, wild type Foxp3-, or an AML1-non binding mutant Foxp3. Sixty hours after transfection, GFP+ cells were sorted and lysed for RNA samples.
Growth protocol Naive CD4+ T cells were activated by soluble anti-CD3 Ab [0.5ug/ml], IL-2 [20u/ml] and antigen presenting cells (T cell-depleted, mitomycin-C-treated splenocytes). Twenty-four hours after transfection, retroviral supernatant and polybrene were added and centrifuged for one hour at 3200 rpm. Cells were kept at 37 C, 5% CO2 until they were sorted.
Extracted molecule total RNA
Extraction protocol We performed RNA extraction using QIAGEN's RNeasy micro columns. according to the manufacteur's protocol.
Label biotin
Label protocol One cycle target labelling according to the protocol provided by Affymetrix
 
Hybridization protocol Labelled cRNA (15ug) were hybridized with Mouse Genome 430 2.0 Array for 16 hours, washed, and stained by streptavidin-phycoerthrin.
Scan protocol The arrays were scanned and aquired data were analyzed by Gene chip operating software (GCOS).
Description Refer to Ono, M. et al, Nature 2007 (Supplementary Figure S7).
Data processing Gene chip operating software (GCOS).
 
Submission date Feb 02, 2007
Last update date Aug 28, 2018
Contact name Masahiro Ono
E-mail(s) m.ono@ucl.ac.uk
Organization name Institute of Child Health
Department Immunobiology Unit
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE6939 CD4+ T cells gene-transduced with AML1, wild type Foxp3, and a Foxp3 mutant defective in binding to AML1
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Normalized GCOS signal
ABS_CALL detection call
DETECTION P-VALUE Detection p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 173.9 P 0.000127
AFFX-BioB-M_at 225.5 P 0.000044
AFFX-BioB-3_at 151.6 P 0.000044
AFFX-BioC-5_at 467.7 P 0.000052
AFFX-BioC-3_at 621.3 P 0.000044
AFFX-BioDn-5_at 1171.5 P 0.000044
AFFX-BioDn-3_at 2158.4 P 0.000044
AFFX-CreX-5_at 6692 P 0.000052
AFFX-CreX-3_at 7578.6 P 0.000044
AFFX-DapX-5_at 797.2 P 0.000044
AFFX-DapX-M_at 1660.6 P 0.000052
AFFX-DapX-3_at 2121.5 P 0.000044
AFFX-LysX-5_at 138.7 P 0.000044
AFFX-LysX-M_at 187.5 P 0.000052
AFFX-LysX-3_at 356.8 P 0.000044
AFFX-PheX-5_at 203.7 P 0.000044
AFFX-PheX-M_at 274.2 P 0.000044
AFFX-PheX-3_at 220.4 P 0.000044
AFFX-ThrX-5_at 223.1 P 0.000052
AFFX-ThrX-M_at 337 P 0.000044

Total number of rows: 45101

Table truncated, full table size 1183 Kbytes.




Supplementary file Size Download File type/resource
GSM160042.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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