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Sample GSM160073 Query DataSets for GSM160073
Status Public on Jul 19, 2007
Title basal culture, 14B3, rep 1
Sample type RNA
 
Source name bipotential mouse embryonic liver cell line 14B3, basal culture
Organism Mus musculus
Characteristics Strain: CBA/J x C57Bl/6J
Tissue: cell lines derived from dpc14 embryonic mouse liver
Cell line: 14B3
Culture condition : basal
Biomaterial provider M. Weiss lab at the Institut Pasteur, Paris, France.
Treatment protocol basal culture (Strick-Marchand H, Weiss MC. Inducible differentiation and morphogenesis of bipotential liver cell lines from wild-type mouse embryos. Hepatology. 2002;36:794-804)
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions. RNA had an 18S/28S ratio greater than 1.7, a 260/230 ratio greater than 1.5, and a lack of visual RNA degradation on Agilent 2100 Bioanalyzer electropherograms (Agilent Technologies Inc.).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array 430a . GeneChips were washed and stained in the Affymetrix Fluidics Station 400 (non-upgraded).
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 (non-upgraded).
Description Gene expression data from 14B3 BMEL cells cultured under basal conditions.
Data processing The data were analyzed with the Bioconductor affy (Gautier L, Cope L, Bolstad BM, et al. affy--analysis of Affymetrix GeneChip data at the probe level. Bioinformatics (Oxford, England). 2004;20(3):307-315) package using R 2.3.1 and RMA (Irizarry RA, Hobbs B, Collin F, et al. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics (Oxford, England). 2003;4(2):249-264) as the normalization method.
 
Submission date Feb 02, 2007
Last update date Jul 11, 2007
Contact name Scott Andrew Ochsner
E-mail(s) sochsner@bcm.edu
Phone 713-798-6227
Organization name Baylor College of Medicine
Department Molecular and Cellular Biology
Lab SPP: Signaling Pathways Project
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL339
Series (2)
GSE6942 Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers (430A)
GSE6966 Transcriptional profiling of bipotential embryonic liver cells to identify liver progenitor cell surface markers.

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1415670_at 1115.745289
1415671_at 769.2346821
1415672_at 2252.529055
1415673_at 1763.637081
1415674_a_at 1018.407335
1415675_at 819.3039727
1415676_a_at 3786.441699
1415677_at 302.3160995
1415678_at 1908.041718
1415679_at 1193.998244
1415680_at 2174.114188
1415681_at 1290.011311
1415682_at 923.0775199
1415683_at 3651.341776
1415684_at 389.37272
1415685_at 353.6648428
1415686_at 1004.888001
1415687_a_at 2153.440878
1415688_at 2111.948588
1415689_s_at 634.9961348

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM160073.CEL.gz 3.6 Mb (ftp)(http) CEL

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