|
| Status |
Public on Feb 04, 2015 |
| Title |
Homo_N1-2 |
| Sample type |
RNA |
| |
|
| Source name |
Adjacent normal brain tissue
|
| Organism |
Homo sapiens |
| Characteristics |
individual: patient 1
tissue: Adjacent normal brain tissue
gender: male
age(years): 59
|
| Treatment protocol |
The tissues were obtained from the operation room and were snap-frozen immediately, stored at -80℃.
|
| Growth protocol |
A totle of 3 GBM tissues and the matched adjacent normal brain tissues were obtained from patients who underwent primary surgical treatment according to clinical protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Human Transcriptome Array, 1 ug of cRNA were hybridized for 16 hr at 48℃ on Affymetrix GeneChip miRNA Array were washed and stained in the affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from Adjacent normal brain tissue.
|
| Data processing |
The data were analyzed with Affymetrix ExpressionConsole-1_4_0 (EC 5.0) using Robust Multichip Average (RMA) as normalization method.
|
| |
|
| Submission date |
Feb 04, 2015 |
| Last update date |
Feb 04, 2015 |
| Contact name |
Weitao Man |
| E-mail(s) |
manweitao@qq.com
|
| Phone |
+8618201060638
|
| Organization name |
Fuxing Hospital affiliated to Capital Medical University
|
| Street address |
Fuxing Hospital affiliated to Capital Medical University,Beijing,China
|
| City |
Beijing |
| ZIP/Postal code |
100036 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE65626 |
Expression data from glioblastoma tissue and matched adjacent normal tissue |
|
