|
| Status |
Public on Feb 07, 2015 |
| Title |
Uninfected-Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293T_uninfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
infected with: none (uninfected)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNA extraction using miRVana miRNA isolation Kit.
|
| Label |
biotin
|
| Label protocol |
omplex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
500 ng RNA were hybridised for 16h at 60 rpm at 48°C on Affymetrix GeneChip miRNA 4.0 Array and scanned by Affymetrix 3000 7G.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
SAMPLE 1.rma-dabg-Signal
|
| Data processing |
Scanned images of microarray chips were analyzed using Affymetrix Expression Console with the default RMA settings. Raw data were processed and Nromalized on basis of organism human using RMA algorithm. The normalized data (CHP files) processed for the differential miRNA analysis in Affymetrix TAC software.
|
| |
|
| Submission date |
Feb 06, 2015 |
| Last update date |
Feb 07, 2015 |
| Contact name |
Himanshu Kumar |
| E-mail(s) |
hkumar@iiserb.ac.in
|
| Organization name |
IISER Bhopal
|
| Department |
Biological Sciences
|
| Lab |
215
|
| Street address |
Indore bypass road
|
| City |
Bhopal |
| State/province |
MADHYA PRADESH |
| ZIP/Postal code |
462066 |
| Country |
India |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE65694 |
MicroRNA profiling in HEK293T cells after NDV infection |
|
