|
| Status |
Public on Feb 16, 2004 |
| Title |
Untreated B cells 60min vs Anti-IgM treated B cells 60min (set 4) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from untreated B cells cultured for 60min was labeled with Cyanine-3 (green).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
Total RNA from B cells treated with Anti-IgM for 60min was labeled with Cyanine-5 (red).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Control total RNA was extracted from untreated B cells cultured for 60min.
Test total RNA was extracted from B cells treated with Anti-IgM for 60min.
|
| |
|
| Submission date |
Feb 02, 2004 |
| Last update date |
May 28, 2005 |
| Contact name |
Sangdun Choi |
| E-mail(s) |
schoi@its.caltech.edu
|
| Phone |
626-395-8732
|
| URL |
http://www.signaling-gateway.org
|
| Organization name |
California Institute of Technology
|
| Department |
Biology
|
| Lab |
Alliance for Cellular Signaling Molecular Biology Laboratory
|
| Street address |
1200 E. California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL260 |
| Series (3) |
| GSE1014 |
B cell response to CD40, Anti-IgM, ELC, IL4, terbutaline and lipopolysaccharide treatment |
| GSE1019 |
B cell response to Anti-IgM and CD40 treatment |
| GSE1021 |
B cell response to Anti-IgM and terbutaline treatment |
|
| Data table header descriptions |
| ID_REF |
Feature number which correspond to the order of genes on the array |
| G_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computing the log ratio |
| G_MEAN |
Green Channel Mean Raw Intensity |
| G_MEDIAN |
Green Channel Median Raw Intensity |
| G_SD |
Green Channel Standard Deviation |
| G_BG_MEAN |
Green Channel Mean Background Level |
| G_BG_MEDIAN |
Green Channel Median Background Level |
| G_BG_SD |
Green Channel Background Standard Deviation |
| R_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computing the log ratio |
| R_MEAN |
Red Channel Mean Raw Intensity |
| R_MEDIAN |
Red Channel Median Raw Intensity |
| R_SD |
Red Channel Standard Deviation |
| R_BG_MEAN |
Red Channel Mean Background Level |
| R_BG_MEDIAN |
Red Channel Median Background Level |
| R_BG_SD |
Red Channel Background Standard Deviation |
| RATIO |
Log Ratio (base 10) |
| VALUE |
Processed Ratio (base 2) after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots. |
