|
| Status |
Public on Mar 05, 2007 |
| Title |
FOG-1-independent transcripition by GATA-1(V205G) in G1E cells, 0h |
| Sample type |
RNA |
| |
|
| Source name |
G1E cells expressing ER-GATA-1(V205G); prior to estradiol treatment
|
| Organism |
Mus musculus |
| Characteristics |
GATA-1-null G1E erythroid precursor cells expressing a GATA-1 V205G mutant as a fusion to the estrogen receptor ligand binding domain.
|
| Biomaterial provider |
G1E cells were provided by Mitchell J. Weiss
|
| Treatment protocol |
samples represent untreated controls for comarison to cells treated for 24 h with 1 uM estradiol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Trizol from up 10 million cells. Further purification was achieved via Qiagen RNeasy column purification.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol. University of Chicago Microarray Facility
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol. University of Chicago Microarray Facility
|
| Scan protocol |
Affymetrix standard protocol. University of Chicago Microarray Facility
|
| Description |
Affymetrix standard protocol. University of Chicago Microarray Facility
|
| Data processing |
Affymetrix standard protocol. University of Chicago Microarray Facility
1) normalization method: scale to 500
2) data selection procedures and parameters, transformation algorithm: GCOS default setting
3) scaling parameters: 500
|
| |
|
| Submission date |
Feb 05, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kirby Dean Johnson |
| E-mail(s) |
kjohns25@wisc.edu
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pharmacology
|
| Lab |
Emery H. Bresnick
|
| Street address |
1300 University Avenue
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6958 |
FOG-1-independent transcripition by GATA-1(V205G) in G1E cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
