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Sample GSM160445 Query DataSets for GSM160445
Status Public on Mar 05, 2007
Title FOG-1-independent transcripition by GATA-1(V205G) in G1E cells; 24h
Sample type RNA
 
Source name G1E cells expressing ER-GATA-1(V205G); 24 h estradiol treatment
Organism Mus musculus
Characteristics GATA-1-null G1E erythroid precursor cells expressing a GATA-1 V205G mutant as a fusion to the estrogen receptor ligand binding domain. Treated with 1 uM estradiol for 24 h.
Biomaterial provider G1E cells were provided by Mitchell J. Weiss
Treatment protocol samples represent cells treated for 24 h with 1 uM estradiol.
Extracted molecule total RNA
Extraction protocol RNA was isolated with Trizol from up 10 million cells. Further purification was achieved via Qiagen RNeasy column purification.
Label biotin
Label protocol Affymetrix standard protocol. University of Chicago Microarray Facility
 
Hybridization protocol Affymetrix standard protocol. University of Chicago Microarray Facility
Scan protocol Affymetrix standard protocol. University of Chicago Microarray Facility
Description Affymetrix standard protocol. University of Chicago Microarray Facility
Data processing Affymetrix standard protocol. University of Chicago Microarray Facility
1) normalization method: scale to 500

2) data selection procedures and parameters, transformation algorithm: GCOS default setting

3) scaling parameters: 500
 
Submission date Feb 05, 2007
Last update date Aug 28, 2018
Contact name Kirby Dean Johnson
E-mail(s) kjohns25@wisc.edu
Organization name University of Wisconsin-Madison
Department Pharmacology
Lab Emery H. Bresnick
Street address 1300 University Avenue
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platform ID GPL1261
Series (1)
GSE6958 FOG-1-independent transcripition by GATA-1(V205G) in G1E cells
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal value in GATA-1-null G1E cells; 24 h estradiol treatment
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1435355_at 2.3 A 0.828613
1433933_s_at 10.2 A 0.533936
1422542_at 2.1 A 0.780518
1430762_at 1.6 A 0.953857
1436543_at 2.6 A 0.780518
1424965_at 15.3 A 0.753906
1425470_at 7 A 0.753906
1418809_at 4.7 A 0.72583
1427976_at 3 A 0.850342
1419060_at 495.1 P 0.01416
1460738_at 4.8 A 0.780518
1420255_at 0.2 A 0.991943
1455178_at 52.5 A 0.334473
1420464_s_at 31.1 P 0.016602
1448730_at 1145.7 P 0.00293
1420398_at 17.5 A 0.32666
1421456_at 6.3 A 0.943848
1423895_a_at 27.5 A 0.753906
1442263_at 35.3 A 0.19458
1452815_at 17.3 P 0.046143

Total number of rows: 45101

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM160445.CEL.gz 4.5 Mb (ftp)(http) CEL

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