|
| Status |
Public on Mar 10, 2015 |
| Title |
KURC1 sunitinib-resistance#2 |
| Sample type |
RNA |
| |
|
| Source name |
renal cancer xenograft tumor of KURC1 treated with sunitinib and resected at sunitinib-resistant status
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: xenograft tumor of renal cell carcinoma
treatment: 40 mg/day of sunitinib
xenograft mode: KURC1
|
| Treatment protocol |
We administered 40 mg/day of sunitinib into KURC1, 2, and 3 orally, and evaluated the effect of sunitinib treatment.
|
| Growth protocol |
human renal cancer tissues were transplanted into severe combined immunodefficiency mice and passaged stably. We named them as KURC (Kyoto University Renal Cancer) 1, 2, and 3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated and purified using the Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Biotin Allonamide Triphosphate
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol of GeneChip® WT Terminal Labeling Kit. (GeneChip® WT Terminal Labeling and Hybridization User Manual 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G（Affymetrix）.
|
| Data processing |
The data were analyzed with GeneChip® Command Console® Software（AGCC） and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 26, 2015 |
| Last update date |
Mar 10, 2015 |
| Contact name |
Shibasaki Noboru |
| Organization name |
Kyoto University
|
| Department |
Urology
|
| Street address |
54 Shogoinkawahara-cho Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8561 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE66346 |
Expression data from renal cancer xenograft tumor treated with sunitinib or vehicle |
|
