|
| Status |
Public on Jun 06, 2007 |
| Title |
2.5 microgram total RNA from EPC derived microvescicles s3 |
| Sample type |
RNA |
| |
|
| Source name |
S3, particle RNA: 2.5 microgram
|
| Organism |
Homo sapiens |
| Characteristics |
Microvescicles from human endothelial progenitor cells
|
| Biomaterial provider |
Benedetta Bussolati, Dept. of Internal Medicine, c.so Dogliotti 14, 10126 Torino (Italy)
|
| Treatment protocol |
Microvescicles purified as Ratajczak et al. Leukemia 2006, 20:847
|
| Growth protocol |
Cells are grown as described in Biancone et al. J. Immunol. 2004, 173:5268
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent, as described by manufacturer
|
| Label |
Biotin
|
| Label protocol |
IVT labelling, Illumina® TotalPrep™ RNA Amplification Kit Ambion
|
| |
|
| Hybridization protocol |
Standard protocol suggested from Illumina
|
| Scan protocol |
Standard protocol suggested from Illumina
|
| Description |
None
|
| Data processing |
Average intensities were obtained by BeadStudio 1.5.1.3 with out normalization and background subtraction. Subsequently data were normalized by cyclic loess as described in Bolstad et al. Bioinformatics 2003, 19:185
|
| |
|
| Submission date |
Feb 13, 2007 |
| Last update date |
Jun 06, 2007 |
| Contact name |
Raffaele A Calogero |
| E-mail(s) |
raffaele.calogero@unito.it
|
| Phone |
++39 0116706454
|
| Organization name |
University of Torino
|
| Department |
Molecular Biotechnology Center
|
| Lab |
Bioinformatics and Genomics Unit
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
To |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE7019 |
Total RNA from EPC derived microvescicles |
|
