|
Status |
Public on Jun 06, 2007 |
Title |
0.25 microgram total RNA from EPC derived microvescicles s6 |
Sample type |
RNA |
|
|
Source name |
S6, particle RNA: 0.25 microgram
|
Organism |
Homo sapiens |
Characteristics |
Microvescicles from human endothelial progenitor cells.
|
Biomaterial provider |
Benedetta Bussolati, Dept. of Internal Medicine, c.so Dogliotti 14, 10126 Torino (Italy)
|
Treatment protocol |
Microvescicles purified as Ratajczak et al. Leukemia 2006, 20:847
|
Growth protocol |
Cells are grown as described in Biancone et al. J. Immunol. 2004, 173:5268
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol reagent, as described by manufacturer
|
Label |
Biotin
|
Label protocol |
IVT labelling, Illumina® TotalPrep™ RNA Amplification Kit Ambion
|
|
|
Hybridization protocol |
Standard protocol suggested from Illumina
|
Scan protocol |
Standard protocol suggested from Illumina
|
Description |
None
|
Data processing |
Average intensities were obtained by BeadStudio 1.5.1.3 with out normalization and background subtraction. Subsequently data were normalized by cyclic loess as described in Bolstad et al. Bioinformatics 2003, 19:185
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|
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Submission date |
Feb 13, 2007 |
Last update date |
Jun 06, 2007 |
Contact name |
Raffaele A Calogero |
E-mail(s) |
raffaele.calogero@unito.it
|
Phone |
++39 0116706454
|
Organization name |
University of Torino
|
Department |
Molecular Biotechnology Center
|
Lab |
Bioinformatics and Genomics Unit
|
Street address |
Via Nizza 52
|
City |
Torino |
State/province |
To |
ZIP/Postal code |
10126 |
Country |
Italy |
|
|
Platform ID |
GPL2507 |
Series (1) |
GSE7019 |
Total RNA from EPC derived microvescicles |
|