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Sample GSM1623227 Query DataSets for GSM1623227
Status Public on Apr 07, 2015
Title UNC93B-/- FLU_16h
Sample type RNA
 
Source name UNC93B-/-_PBMC_FLU_16h
Organism Homo sapiens
Characteristics subject status: patients with deficiencies for UNC93B
cell type: PBMC
infected with: influenza virus A/CA/4/2009 for 16hrs
Treatment protocol 0.5x10^6 PBMCs were washed twice with PBS and infected with influenza A/California/4/2009 virus at MOI=2 in RPMI containing 0.3% bovine serum albumin and 1ug/mL TPCK-treated trypsin.
Growth protocol PBMCs were cultured in RPMI 16-40 medium with 10% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Qiagen Rneasy mini kit, followed by DNase I treatment and clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent 2100 Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA targets were prepared from 250 ng of total RNA, using the Illumina TotalPrep RNA Amplification kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description SAMPLE 15
Data processing The data were normalised using quantile normalisation with in R. PALO (p<0.05 present in at least one) were used to filter the data.
 
Submission date Mar 03, 2015
Last update date Jan 03, 2024
Contact name Stephanie Osmond
E-mail(s) sosmond@benaroyaresearch.org
Organization name Benaroya Research Institute
Street address 1201 9th Ave
City Seattle,
State/province WA
ZIP/Postal code 98101
Country USA
 
Platform ID GPL10558
Series (1)
GSE66486 Response of IRF7-deficient peripheral blood mononuclear cells to pH1N1 influenza virus infection

Data table header descriptions
ID_REF
VALUE Quantile normalized

Data table
ID_REF VALUE
ILMN_3241953 4.975349994
ILMN_1745607 3.321928095
ILMN_1735045 3.321928095
ILMN_1680754 3.321928095
ILMN_1755321 3.336852866
ILMN_1698554 4.295509944
ILMN_1814092 3.321928095
ILMN_2061446 6.401954034
ILMN_1676336 6.019201667
ILMN_3237396 7.284852898
ILMN_1688755 5.990166128
ILMN_1653165 6.376881027
ILMN_1662364 10.08007111
ILMN_1674698 6.736743139
ILMN_1700461 6.791457233
ILMN_1784269 6.230956362
ILMN_1698189 6.816799754
ILMN_2096191 8.14019287
ILMN_1678323 3.321928095
ILMN_2157219 3.321928095

Total number of rows: 20517

Table truncated, full table size 499 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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