|
| Status |
Public on Apr 07, 2015 |
| Title |
IRF7-/- FLU_8h |
| Sample type |
RNA |
| |
|
| Source name |
IRF7-/-_PBMC_FLU_8h
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: patients with deficiencies for IRF7
cell type: PBMC
infected with: influenza virus A/CA/4/2009 for 8hrs
|
| Treatment protocol |
0.5x10^6 PBMCs were washed twice with PBS and infected with influenza A/California/4/2009 virus at MOI=2 in RPMI containing 0.3% bovine serum albumin and 1ug/mL TPCK-treated trypsin.
|
| Growth protocol |
PBMCs were cultured in RPMI 16-40 medium with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Qiagen Rneasy mini kit, followed by DNase I treatment and clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA targets were prepared from 250 ng of total RNA, using the Illumina TotalPrep RNA Amplification kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 17
|
| Data processing |
The data were normalised using quantile normalisation with in R. PALO (p<0.05 present in at least one) were used to filter the data.
|
| |
|
| Submission date |
Mar 03, 2015 |
| Last update date |
Jan 03, 2024 |
| Contact name |
Stephanie Osmond |
| E-mail(s) |
sosmond@benaroyaresearch.org
|
| Organization name |
Benaroya Research Institute
|
| Street address |
1201 9th Ave
|
| City |
Seattle, |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE66486 |
Response of IRF7-deficient peripheral blood mononuclear cells to pH1N1 influenza virus infection |
|
