|
| Status |
Public on Mar 06, 2015 |
| Title |
CD3-CD56dimCD57brightNKG2A-NKG2C-, biological replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
CD3-CD56dimCD57brightNKG2A-NKG2C-
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: donor 3
tissue: PBMC
cell subset: CD57brightNKG2A-NKG2C-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolate with Rneasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Following labeling, 500 ng of cDNA were hybridized at 45°C for 16 hr
|
| Scan protocol |
Affymetrix Gene Chip Scanner 3000 7G
|
| Description |
TH09_E051_P7
|
| Data processing |
Analyses were performed with Affymetrix® Transcriptome Analysis Console (TAC) Software, using a gene-level normalization and signal summarization protocol
probe group file: HTA-2_0.r1.pgf
meta-probeset file: HTA-2_0.na33.1.hg19.transcript.csv
RMA normalized gene level expression values from Affymetrix® Transcriptome Analysis Console (TAC) Software
|
| |
|
| Submission date |
Mar 05, 2015 |
| Last update date |
Mar 06, 2015 |
| Contact name |
Yenan Bryceson |
| Organization name |
Karolinska Institutet
|
| Department |
Department of Medicine
|
| Lab |
CIM, F59
|
| Street address |
Karolinska University Hospital Huddinge
|
| City |
Stockholm |
| State/province |
Sweden |
| ZIP/Postal code |
14186 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE66563 |
Expression analysis of canonical and adaptive human NK cell subsets |
| GSE66564 |
Epigenetic and transcriptional characterisation of CMV driven diversification of NK cells |
|
