|
Status |
Public on May 01, 2015 |
Title |
CP70-Vector-1 |
Sample type |
RNA |
|
|
Source name |
CP70 cells transfected with empty vector, clone-1
|
Organism |
Homo sapiens |
Characteristics |
cell line: ovarian cancer cell line A2780-CP70 protocol: control
|
Growth protocol |
Cells were grown in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), and maintained in a humidified atmosphere with 5% CO2 at 37°C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Amplified and biotinylated sense-strand DNA were prepared according to the standard Affymetrix protocol from 100ng total RNA
|
|
|
Hybridization protocol |
Following fragment and label, 5.2 g of single-strand DNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human transcriptome array 2.0. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from CP70 cells transfected with empty vector
|
Data processing |
affymetrix-algorithm-name = rma-gene-full affymetrix-algorithm-version = 1.0
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|
|
Submission date |
Mar 06, 2015 |
Last update date |
May 01, 2015 |
Contact name |
Qi-En Wang |
E-mail(s) |
qi-en.wang@osumc.edu
|
Phone |
614-292-9012
|
Organization name |
The Ohio State University
|
Department |
Radiation Oncology
|
Street address |
420 W.12th Avenue
|
City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43210 |
Country |
USA |
|
|
Platform ID |
GPL17586 |
Series (1) |
GSE66636 |
DDB2 regulates gene expression in ovarian cancer cell lines |
|