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Sample GSM1633341 Query DataSets for GSM1633341
Status Public on Mar 13, 2015
Title U937_vec DMSO replicate 1
Sample type RNA
Source name U937_vec cells, 24 h DMSO treatment
Organism Homo sapiens
Characteristics cell line: U937
protocol: empty vector
agent: DMSO
Treatment protocol Logarithmically growing cells were treated with 1µM all-trans retinoic acid (ATRA) or an equivalent amount of solvent (DMSO) for 24 h
Growth protocol RPMI 10% FCS 1% Penicillin-Streptomycin-Glutamin
Extracted molecule total RNA
Extraction protocol RNeasy (Qiagen)
Label Biotin
Label protocol The Affymetrix GeneChip 3’ IVT Express Protocol was used for the preparation of biotinylated amplified RNA (aRNA) from 250ng of total RNA.
Hybridization protocol 12.5 µg of fragmented and labeled aRNA were hybridized to Affymetrix Human Affymetrix GeneChip Human Genome U133 plus 2.0 arrays for 16 h at 45 °C in a GeneChip hybridization oven 640.
Scan protocol GeneChips were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000 7G. Fluidics and scan functions were controlled the by the Affymetrix GeneChip Command Console software v3.2 (AGCC).
Data processing Gene level normalized expression signals were calculated from Affymetrix CEL files using the RMA algorithm (Irizarry et al., 2003) in the Affymetrix GeneChip Expression Console v1.3 software.
Submission date Mar 13, 2015
Last update date Mar 13, 2015
Contact name Rotraud Wieser
Organization name Medical University of Vienna
Department Clinic of Medicine I
Street address Waehringer Guertel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
Platform ID GPL570
Series (1)
GSE66854 The oncogene EVI1 enhances transcriptional and biological responses of human myeloid cells to all-trans retinoic acid [U937]

Data table header descriptions
VALUE log2 RMA signal intensity.

Data table
1552563_a_at 3.19
1552829_at 4.88
1552867_at 3.29
1552961_at 4.92
1552972_at 2.31
1552974_at 2.38
1552975_x_at 2.63
1552976_at 5.08
1553083_at 4.93
1553208_s_at 3.63
1553269_at 2.82
1553275_s_at 2.55
1553355_at 3.70
1553356_at 3.09
1553398_at 3.47
1553439_at 5.66
1553456_at 3.79
1553462_at 2.59
1553498_at 4.09
1553547_at 3.17

Total number of rows: 54675

Table truncated, full table size 848 Kbytes.

Supplementary file Size Download File type/resource
GSM1633341_A1_U937_vec_DMSO.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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