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Sample GSM1633352 Query DataSets for GSM1633352
Status Public on Mar 13, 2015
Title U937_EVI1 ATRA replicate 3
Sample type RNA
 
Source name U937_EVI1 cells, 24 h ATRA treatment
Organism Homo sapiens
Characteristics cell line: U937
protocol: EVI1 expression vector
agent: ATRA
Treatment protocol Logarithmically growing cells were treated with 1µM all-trans retinoic acid (ATRA) or an equivalent amount of solvent (DMSO) for 24 h
Growth protocol RPMI 10% FCS 1% Penicillin-Streptomycin-Glutamin
Extracted molecule total RNA
Extraction protocol RNeasy (Qiagen)
Label Biotin
Label protocol The Affymetrix GeneChip 3’ IVT Express Protocol was used for the preparation of biotinylated amplified RNA (aRNA) from 250ng of total RNA.
 
Hybridization protocol 12.5 µg of fragmented and labeled aRNA were hybridized to Affymetrix Human Affymetrix GeneChip Human Genome U133 plus 2.0 arrays for 16 h at 45 °C in a GeneChip hybridization oven 640.
Scan protocol GeneChips were washed and stained in an Affymetrix Fluidics Station FS450, and the fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000 7G. Fluidics and scan functions were controlled the by the Affymetrix GeneChip Command Console software v3.2 (AGCC).
Data processing Gene level normalized expression signals were calculated from Affymetrix CEL files using the RMA algorithm (Irizarry et al., 2003) in the Affymetrix GeneChip Expression Console v1.3 software.
 
Submission date Mar 13, 2015
Last update date Mar 13, 2015
Contact name Rotraud Wieser
E-mail(s) rotraud.wieser@meduniwien.ac.at
Organization name Medical University of Vienna
Department Clinic of Medicine I
Street address Waehringer Guertel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platform ID GPL570
Series (1)
GSE66854 The oncogene EVI1 enhances transcriptional and biological responses of human myeloid cells to all-trans retinoic acid [U937]

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity.

Data table
ID_REF VALUE
1552563_a_at 2.64
1552829_at 5.00
1552867_at 3.94
1552961_at 4.54
1552972_at 2.37
1552974_at 2.41
1552975_x_at 2.40
1552976_at 5.08
1553083_at 5.00
1553208_s_at 3.55
1553269_at 2.84
1553275_s_at 2.48
1553355_at 3.37
1553356_at 3.15
1553398_at 3.29
1553439_at 5.26
1553456_at 3.87
1553462_at 2.91
1553498_at 3.39
1553547_at 2.97

Total number of rows: 54675

Table truncated, full table size 848 Kbytes.




Supplementary file Size Download File type/resource
GSM1633352_C4_U937_EVI1_ATRA.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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