|
| Status |
Public on Nov 19, 2015 |
| Title |
Meioc-/- testes at 8dpp, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
testes, Meioc-/-
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: NMRI
genotype/variation: Meioc-/-
tissue: testes
age: 8 dpp
gender: male
|
| Treatment protocol |
Pairs of fetal ovaries/post-natal testes were dissected from embryos/mouse that were previously weighed. Gonads were dissolved in 50µl RLT lysis buffer (Qiagen) and stored at -20°C before pools constitution. For males, testes were weighed and contralateral testes were fixed in Formol for histological analysis.
|
| Growth protocol |
Mice were breeded in conventional animal facility in accordance with animal health care, ethics and current regulations.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Gonads in RLT buffer were pooled and lysed with homogenizer before RNA extraction using RNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (following the Affymetrix GeneChip Whole Transcript (WT) Sense Target Labelling Assay kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on Affymetrix Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix).
|
| Description |
T8_A-/-
|
| Data processing |
The data were preprocessed and analyzed using Partek Genomic Suite.
|
| |
|
| Submission date |
Mar 13, 2015 |
| Last update date |
Nov 19, 2015 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE66880 |
Expression data from gonads of Meioc+/+ and Meioc-/-mice |
|
