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Sample GSM1648023 Query DataSets for GSM1648023
Status Public on Apr 02, 2015
Title Idiopathic Dilated Cardiomyopathy 1
Sample type RNA
 
Source name IDC_heart
Organism Homo sapiens
Characteristics subject status: idiopathic dilated cardiomyopathy (IDC)
tissue: heart right ventricular wall
Growth protocol Obtained from patients at autopsy
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description IDCC 1
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Apr 01, 2015
Last update date Apr 02, 2015
Contact name James West
E-mail(s) j.west@vanderbilt.edu
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
 
Platform ID GPL6244
Series (1)
GSE67492 Expression data from human heart right ventricular wall

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity (log2)

Data table
ID_REF VALUE
8102800 4.148517
8021635 5.397537
8095744 6.229135
7951271 5.058832
8054712 4.387173
8095736 6.175058
7924029 5.75716
8176719 10.98411
8095728 5.943765
8147351 4.554452
7955613 6.074129
7990391 4.872119
8003298 6.761336
8048864 4.835896
8141016 5.040205
8158167 6.396653
8026490 4.987148
8148040 6.778451
7950899 5.752769
7996837 5.002469

Total number of rows: 28763

Table truncated, full table size 474 Kbytes.




Supplementary file Size Download File type/resource
GSM1648023_IDCC1-HuGene.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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