|
| Status |
Public on Apr 02, 2015 |
| Title |
PAH with BMPR2 mutation 2 |
| Sample type |
RNA |
| |
|
| Source name |
PAH_heart
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: pulmonary arterial hypertension (PAH) with BMPR2 mutation
tissue: heart right ventricular wall
|
| Growth protocol |
Obtained from patients at autopsy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
SPH
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Apr 01, 2015 |
| Last update date |
Apr 02, 2015 |
| Contact name |
James West |
| E-mail(s) |
j.west@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Pulmonary
|
| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE67492 |
Expression data from human heart right ventricular wall |
|
