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Sample GSM1648755 Query DataSets for GSM1648755
Status Public on Jan 03, 2017
Title Retina, 4 hours, euglycemic clamp, replicate 1
Sample type RNA
 
Source name Retina
Organism Mus musculus
Characteristics strain: C57BL/6
gender: female
tissue: retina
age: 2 months
Extracted molecule polyA RNA
Extraction protocol Not provided
Label biotin
Label protocol RNA samples isolated from retina, 4 and 48 h after the end of the clamp (3 samples per condition; 12 samples total), were diluted to 100 ng/μl and used to perform the target preparation using the Whole Transcript Sense Target Labeling Protocol procedure (Affymetrix, High Wycombe, UK).
 
Hybridization protocol 5.5 μg of each fragmented cDNA were end-labeled with biotin and hybridized to a GeneChip Mouse Gene 1.0 ST array (Affymetrix)
Scan protocol U133 Plus 2.0 Arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix)
Data processing Background subtraction, quantile normalization and probeset summarization was performed using the Affymetrix Power Tools apt-probeset-summarize command with the -rma option
 
Submission date Apr 02, 2015
Last update date Jan 03, 2017
Contact name Mark Ibberson
Organization name SIB Swiss Institute of Bioinformatics
Department Vital-IT
Street address Genopode building
City Lausanne
ZIP/Postal code CH-1015
Country Switzerland
 
Platform ID GPL10740
Series (1)
GSE67523 Biological characterization of gene response to insulin-induced hypoglycemia in mouse retina

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
10338001 11.94272
10338002 6.56205
10338003 10.14279
10338004 9.28908
10338005 2.37077
10338006 2.5844
10338007 2.85083
10338008 3.6916
10338009 8.63559
10338010 2.42912
10338011 5.77337
10338012 2.48221
10338013 2.27514
10338014 2.32247
10338015 2.29251
10338016 7.85325
10338017 13.27681
10338018 6.9318
10338019 5.19371
10338020 8.4874

Total number of rows: 241576

Table truncated, full table size 4013 Kbytes.




Supplementary file Size Download File type/resource
GSM1648755_RR4.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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