|
| Status |
Public on Jan 03, 2017 |
| Title |
Retina, 48 hours, hypoglycemic clamp, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Retina
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
tissue: retina
age: 2 months
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Not provided
|
| Label |
biotin
|
| Label protocol |
RNA samples isolated from retina, 4 and 48 h after the end of the clamp (3 samples per condition; 12 samples total), were diluted to 100 ng/μl and used to perform the target preparation using the Whole Transcript Sense Target Labeling Protocol procedure (Affymetrix, High Wycombe, UK).
|
| |
|
| Hybridization protocol |
5.5 μg of each fragmented cDNA were end-labeled with biotin and hybridized to a GeneChip Mouse Gene 1.0 ST array (Affymetrix)
|
| Scan protocol |
U133 Plus 2.0 Arrays were scanned using the GeneChip® Scanner 3000 7G (Affymetrix)
|
| Data processing |
Background subtraction, quantile normalization and probeset summarization was performed using the Affymetrix Power Tools apt-probeset-summarize command with the -rma option
|
| |
|
| Submission date |
Apr 02, 2015 |
| Last update date |
Jan 03, 2017 |
| Contact name |
Mark Ibberson |
| Organization name |
SIB Swiss Institute of Bioinformatics
|
| Department |
Vital-IT
|
| Street address |
Genopode building
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE67523 |
Biological characterization of gene response to insulin-induced hypoglycemia in mouse retina |
|
