|
| Status |
Public on Feb 18, 2004 |
| Title |
1hHypo_3hReox_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HUVEC with 1 hour hypoxia and 3 hours reoxygenation
|
| Organism |
Homo sapiens |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
Human universal reference RNA (Stratagene, La Jolla, CA)
|
| Organism |
Homo sapiens |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Human umbilical vein endothelial cells (HUVEC) were cultivated on 1%-gelatin-coated 75-cm2 culture flasks in Medium 200 supplemented with 2% fetal calf serum, penicillin (100 units/ml), streptomycin (100 units/ml) and Funizone (0.25 μg/ml) supplied by Cascade Biologics (Portland, OR). Prior to an experiment, HUVECs were subcultivated with Trypsin/EDTA onto 1% gelatin coated glass 30-mm glass coverslips in 60 mm glass Petri dishes.
|
| |
|
| Submission date |
Feb 11, 2004 |
| Last update date |
Sep 08, 2006 |
| Contact name |
Phillip Bowman |
| E-mail(s) |
phillip.bowman@cen.amedd.army.mil
|
| Phone |
210-916-4297
|
| Organization name |
Institute of Surgical Research
|
| Street address |
3400 Rawley E. Chambers Ave
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78234-6315 |
| Country |
USA |
| |
|
| Platform ID |
GPL981 |
| Series (1) |
| GSE1041 |
Responses of HUVEC to Hypoxia and Reoxygenation |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
same as UNF_VALUE but with flagged values removed |
| F635_MEDIAN |
Cy5 channel median intensity of the spot. |
| F635_MEAN |
Cy5 channel average intensity of the spot. |
| F635_SD |
The standard deviation calculated for the Cy5 channel mean intensity of the spot. |
| B635_MEDIAN |
Cy5 channel background median intensity around the spot. |
| B635_MEAN |
Cy5 channel background average intensity around the spot. |
| B635_SD |
The standard deviation calculated for the Cy5 channel background average intensity around the spot. |
| F635_QUALITY |
Cy5 channel percentage of pixels in the spot more than one standard deviation above background. |
| F532_MEDIAN |
Cy3 channel median intensity of the spot. |
| F532_MEAN |
Cy3 channel average intensity of the spot. |
| F532_SD |
The standard deviation calculated for the Cy3 channel mean intensity of the spot. |
| B532_MEDIAN |
Cy3 channel background median intensity around the spot. |
| B532_MEAN |
B532_MEDIAN: Cy3 channel background median intensity around the spot. |
| B532_SD |
The standard deviation calculated for the Cy3 channel background average intensity around the spot. |
| F532_QUALITY |
Cy3 channel percentage of pixels in the spot more than one standard deviation above background. |
| FLAGS |
The indication of spot quality determined by the image analysis software. Unflaged spot was assigned a value of zero. A negative value was assigned to a spot with intensity near background, uneven spot or background intensity. |
| UNF_VALUE |
log ratio of Median of Ratios between Cy5 and Cy3 channels. (log2 of Median of Ratios). The Median of Ratios is the median of the pixel-by-pixel ratios of pixel intensities that have had the median background intensity subtracted. |
