|
Status |
Public on May 20, 2015 |
Title |
WT_sedentary_rep3 |
Sample type |
RNA |
|
|
Source name |
gastrocnemius muscle
|
Organism |
Mus musculus |
Characteristics |
tissue: gastrocnemius muscle genotype: WT exercise: sedentary
|
Treatment protocol |
Exercise endurance was determined using a Columbus Instruments modular treadmill. Eight mice could be run at a time in this system. Prior to the measurement, all mice were weighed and allowed to orient to the inclined treadmill (10o) surroundings for 3-5 minutes. A ramping program was then begun at 4 m/min for 5 min to allow the animals to acclimate to the movement of the belt and orient them to the shock grid. The speed was increased to 10 m/min for 10 min and then increased 2 m/min every 2 minutes (to a maximum speed of 40 m/min) until the animals run to exhaustion.
|
Growth protocol |
Mice were fed a high fat diet consisting of high-fat, high-sucrose diet (HFHSD, D12331 Research Diet) starting at 8-10 weeks of age for 14-18 weeks.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from flash-frozen mouse tissue samples by using Trizol (Invitrogen) isolation method and cleaned up with RNAEasy mini columns (Qiagen). cDNA was produced using a high capacity cDNA transcription kit.
|
Label |
biotin
|
Label protocol |
Gene expression profiles were obtained from Affymetrics GeneChip Mouse Genome 430 2.0 Arrays. The microarray data was first preprocessed, including background correction and normalization, by Robust Multi-array Average (RMA) with custom CDF downloaded from BrainArray
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|
|
Hybridization protocol |
The biotinylated cRNA was prepared and hybridized to the Mouse 430A_2.0 GeneChip in accordance with Affymetrix protocols.
|
Scan protocol |
GeneChips were scanned by the Affymetrix GeneChip Scanner.
|
Description |
WT_sedentary_rep3
|
Data processing |
Microarray data was preprocessed using RMA implemented in R with BrainArray custom CDF.
|
|
|
Submission date |
May 19, 2015 |
Last update date |
May 20, 2015 |
Contact name |
Jing Chen |
Organization name |
CCHMC
|
Street address |
3333 Burnet Ave
|
City |
Cincinnati |
State/province |
OH |
ZIP/Postal code |
45229 |
Country |
USA |
|
|
Platform ID |
GPL19559 |
Series (1) |
GSE69062 |
Genetic ablation of CD38 protects against Western diet-induced exercise intolerance and metabolic inflexibility |
|