|
| Status |
Public on May 20, 2015 |
| Title |
KO_sedentary_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius muscle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: gastrocnemius muscle
genotype: CD38 KO
exercise: sedentary
|
| Treatment protocol |
Exercise endurance was determined using a Columbus Instruments modular treadmill. Eight mice could be run at a time in this system. Prior to the measurement, all mice were weighed and allowed to orient to the inclined treadmill (10o) surroundings for 3-5 minutes. A ramping program was then begun at 4 m/min for 5 min to allow the animals to acclimate to the movement of the belt and orient them to the shock grid. The speed was increased to 10 m/min for 10 min and then increased 2 m/min every 2 minutes (to a maximum speed of 40 m/min) until the animals run to exhaustion.
|
| Growth protocol |
Mice were fed a high fat diet consisting of high-fat, high-sucrose diet (HFHSD, D12331 Research Diet) starting at 8-10 weeks of age for 14-18 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from flash-frozen mouse tissue samples by using Trizol (Invitrogen) isolation method and cleaned up with RNAEasy mini columns (Qiagen). cDNA was produced using a high capacity cDNA transcription kit.
|
| Label |
biotin
|
| Label protocol |
Gene expression profiles were obtained from Affymetrics GeneChip Mouse Genome 430 2.0 Arrays. The microarray data was first preprocessed, including background correction and normalization, by Robust Multi-array Average (RMA) with custom CDF downloaded from BrainArray
|
| |
|
| Hybridization protocol |
The biotinylated cRNA was prepared and hybridized to the Mouse 430A_2.0 GeneChip in accordance with Affymetrix protocols.
|
| Scan protocol |
GeneChips were scanned by the Affymetrix GeneChip Scanner.
|
| Description |
KO_sedentary_rep3
|
| Data processing |
Microarray data was preprocessed using RMA implemented in R with BrainArray custom CDF.
|
| |
|
| Submission date |
May 19, 2015 |
| Last update date |
May 20, 2015 |
| Contact name |
Jing Chen |
| Organization name |
CCHMC
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL19559 |
| Series (1) |
| GSE69062 |
Genetic ablation of CD38 protects against Western diet-induced exercise intolerance and metabolic inflexibility |
|
