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Sample GSM1692589 Query DataSets for GSM1692589
Status Public on Jul 09, 2015
Title KMS-11/Cfz_rep3_261901
Sample type RNA
 
Source name KMS-11/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
Organism Homo sapiens
Characteristics cell line: KMS-11/Cfz
cell type: multiple myeloma
Treatment protocol KMS-11/Cfz and KMS-34/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental KMS-11 and KMS-34 cells which had not been selected in the drug.
Growth protocol Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the miRNeasy mini kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA was prepared by Expression Analysis, Inc. (Durham, NC).
 
Hybridization protocol Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis, Inc.
Scan protocol Scanning of GeneChip arrays was performed by Expression Analysis, Inc.
Description Gene expression data from KMS-11/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
Data processing The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
 
Submission date May 20, 2015
Last update date Jul 10, 2015
Contact name Robert Hawley
Organization name George Washington University
Department Anatomy and Regenerative Biology
Street address 2300 I Street NW, Suite 431
City Washington
State/province DC
ZIP/Postal code 20037
Country USA
 
Platform ID GPL570
Series (1)
GSE69078 Comparison of the gene expression profiles of carfilzomib-resistant derivatives versus parental human myeloma cell lines

Data table header descriptions
ID_REF
VALUE log2 transformed data

Data table
ID_REF VALUE
1007_s_at 0.20998287
1053_at 0.002162933
117_at 0.51292086
121_at 0.02814579
1255_g_at 0.02668333
1294_at 0.10864353
1316_at 0.23336887
1320_at 0.14905453
1405_i_at 0.5247154
1431_at -0.14052963
1438_at -0.04129696
1487_at -0.7523079
1494_f_at 0.12817001
1598_g_at 0.16340256
160020_at 0.12497902
1729_at -0.23085022
1773_at -0.15814734
177_at 0.14101124
179_at 0.17965412
1861_at -0.06620121

Total number of rows: 54613

Table truncated, full table size 1195 Kbytes.




Supplementary file Size Download File type/resource
GSM1692589_EA13015_261901_HG-U133_PLUS_2_18.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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