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Sample GSM1692590 Query DataSets for GSM1692590
Status Public on Jul 09, 2015
Title KMS-34/Cfz_rep1_261893
Sample type RNA
 
Source name KMS-34/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
Organism Homo sapiens
Characteristics cell line: KMS-34/Cfz
cell type: multiple myeloma
Treatment protocol KMS-11/Cfz and KMS-34/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental KMS-11 and KMS-34 cells which had not been selected in the drug.
Growth protocol Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the miRNeasy mini kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA was prepared by Expression Analysis, Inc. (Durham, NC).
 
Hybridization protocol Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis, Inc.
Scan protocol Scanning of GeneChip arrays was performed by Expression Analysis, Inc.
Description Gene expression data from KMS-34/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
Data processing The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
 
Submission date May 20, 2015
Last update date Jul 10, 2015
Contact name Robert Hawley
Organization name George Washington University
Department Anatomy and Regenerative Biology
Street address 2300 I Street NW, Suite 431
City Washington
State/province DC
ZIP/Postal code 20037
Country USA
 
Platform ID GPL570
Series (1)
GSE69078 Comparison of the gene expression profiles of carfilzomib-resistant derivatives versus parental human myeloma cell lines

Data table header descriptions
ID_REF
VALUE log2 transformed data

Data table
ID_REF VALUE
1007_s_at 0.13493109
1053_at -0.1635971
117_at -0.030361176
121_at 0.07913113
1255_g_at 0.20726109
1294_at -0.04781866
1316_at -0.10402489
1320_at 0.102796555
1405_i_at 0.23682594
1431_at 0.020491123
1438_at 0.16947937
1487_at 0.30598354
1494_f_at -0.08547974
1598_g_at -0.0954504
160020_at 0.25433683
1729_at 0.012212753
1773_at -0.100924015
177_at 0.016330242
179_at 0.14625359
1861_at -0.39226627

Total number of rows: 54613

Table truncated, full table size 1186 Kbytes.




Supplementary file Size Download File type/resource
GSM1692590_EA13015_261893_HG-U133_PLUS_2_10.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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