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Sample GSM1692593 Query DataSets for GSM1692593
Status Public on Jul 09, 2015
Title KMS-11_rep1_261898
Sample type RNA
 
Source name KMS-11 multiple myeloma cells
Organism Homo sapiens
Characteristics cell line: KMS-11
cell type: multiple myeloma
Treatment protocol KMS-11/Cfz and KMS-34/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental KMS-11 and KMS-34 cells which had not been selected in the drug.
Growth protocol Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the miRNeasy mini kit (Qiagen).
Label biotin
Label protocol Biotinylated cRNA was prepared by Expression Analysis, Inc. (Durham, NC).
 
Hybridization protocol Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis, Inc.
Scan protocol Scanning of GeneChip arrays was performed by Expression Analysis, Inc.
Description Gene expression data from KMS-11 multiple myeloma cells
Data processing The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
 
Submission date May 20, 2015
Last update date Jul 10, 2015
Contact name Robert Hawley
Organization name George Washington University
Department Anatomy and Regenerative Biology
Street address 2300 I Street NW, Suite 431
City Washington
State/province DC
ZIP/Postal code 20037
Country USA
 
Platform ID GPL570
Series (1)
GSE69078 Comparison of the gene expression profiles of carfilzomib-resistant derivatives versus parental human myeloma cell lines

Data table header descriptions
ID_REF
VALUE log2 transformed data

Data table
ID_REF VALUE
1007_s_at 0.03210163
1053_at -0.15324402
117_at 0.29171324
121_at 0.1778593
1255_g_at -0.021009207
1294_at 0.19818163
1316_at 0.1477871
1320_at 0.052210808
1405_i_at 0.55772305
1431_at -0.14370203
1438_at 0.019676685
1487_at -0.56243896
1494_f_at -0.12680769
1598_g_at 0.30521584
160020_at 0.16352892
1729_at -0.44599056
1773_at -0.09426832
177_at -0.20304632
179_at 0.3061695
1861_at 0.026789665

Total number of rows: 54613

Table truncated, full table size 1197 Kbytes.




Supplementary file Size Download File type/resource
GSM1692593_EA13015_261898_HG-U133_PLUS_2_15.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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