|
| Status |
Public on May 22, 2015 |
| Title |
IL-4-treated macrophages transfected with miR-142-5p ASO-2 |
| Sample type |
RNA |
| |
|
| Source name |
IL-4-treated macrophages transfected with miR-142-5p ASO
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: healthy donor 2
tissue: buffy coats of peripheral blood
cell type: primary human monocyte-derived macrophages
transfected with: miR-142-5p ASO
treated with: 20ng/ml IL-4 for 24h
|
| Treatment protocol |
Afterward, macrophages were transfected with control ASO, miR-142-5p ASO, control mimics or miR-130-3p mimics using lentiviral vectors. After 24 hr, the cells were treated with 20ng/ml IL-4 for 24h.
|
| Growth protocol |
Peripheral blood monocytes from healthy donors were isolated by Ficoll density gradient centrifugation as previously described. Macrophages were obtained by culturing monocytes in DMEM medium supplemented with 10% heat inactivated human AB serum for 6 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using TRIzol (Invitrogen) mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre).
|
| Label |
Cy3
|
| Label protocol |
each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method
|
| |
|
| Hybridization protocol |
Hybridization was performed by Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242).
|
| Scan protocol |
The slides were scanned with the Agilent DNA Microarray Scanner.
|
| Description |
mRNA expression of IL-4-treated macrophages transfected with miR-142-5p ASO
|
| Data processing |
Data was extracted using Agilent Feature Extraction software. Results were provided in the Raw Intensity.xls file. Normalization was performed using the Agilent GeneSpring GX v11.5.1.
After quantile normalization of the raw data,mRNAs that at least 2 out of 5 samples have flags in Present or Marginal (“All Targets Value”) were chosen for further data analysis. Differentially expressed mRNAs were identified through Fold Change filtering.
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| |
|
| Submission date |
May 22, 2015 |
| Last update date |
May 22, 2015 |
| Contact name |
Shicheng Su |
| E-mail(s) |
seasonso@163.com
|
| Phone |
84-02-87332022
|
| Organization name |
Sun-Yat-Sen Memorial Hospital
|
| Street address |
107 Yanjiang West Road
|
| City |
Guangzhou |
| ZIP/Postal code |
510120 |
| Country |
China |
| |
|
| Platform ID |
GPL13497 |
| Series (2) |
| GSE59347 |
mRNA expression profile in IL-4-treated macrophages transfected with miR-142-5p ASO or miR-130a-3p mimics |
| GSE59349 |
The differential miRNA expression induces M2 macrophage polarization and mediates their profibrogenic effects |
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