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Sample GSM1694105 Query DataSets for GSM1694105
Status Public on May 22, 2015
Title IL-4-treated macrophages transfected with control ASO-2
Sample type RNA
 
Source name IL-4-treated macrophages transfected with control ASO
Organism Homo sapiens
Characteristics donor id: healthy donor 2
tissue: buffy coats of peripheral blood
cell type: primary human monocyte-derived macrophages
transfected with: control ASO
treated with: 20ng/ml IL-4 for 24h
Treatment protocol Afterward, macrophages were transfected with control ASO, miR-142-5p ASO, control mimics or miR-130-3p mimics using lentiviral vectors. After 24 hr, the cells were treated with 20ng/ml IL-4 for 24h.
Growth protocol Peripheral blood monocytes from healthy donors were isolated by Ficoll density gradient centrifugation as previously described. Macrophages were obtained by culturing monocytes in DMEM medium supplemented with 10% heat inactivated human AB serum for 6 days.
Extracted molecule total RNA
Extraction protocol Total RNA was harvested using TRIzol (Invitrogen) mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre).
Label Cy3
Label protocol each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method
 
Hybridization protocol Hybridization was performed by Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242).
Scan protocol The slides were scanned with the Agilent DNA Microarray Scanner.
Description mRNA expression of IL-4-treated macrophages transfected with control ASO
Data processing Data was extracted using Agilent Feature Extraction software. Results were provided in the Raw Intensity.xls file. Normalization was performed using the Agilent GeneSpring GX v11.5.1.
After quantile normalization of the raw data,mRNAs that at least 2 out of 5 samples have flags in Present or Marginal (“All Targets Value”) were chosen for further data analysis. Differentially expressed mRNAs were identified through Fold Change filtering.
 
Submission date May 22, 2015
Last update date May 22, 2015
Contact name Shicheng Su
E-mail(s) seasonso@163.com
Phone 84-02-87332022
Organization name Sun-Yat-Sen Memorial Hospital
Street address 107 Yanjiang West Road
City Guangzhou
ZIP/Postal code 510120
Country China
 
Platform ID GPL13497
Series (2)
GSE59347 mRNA expression profile in IL-4-treated macrophages transfected with miR-142-5p ASO or miR-130a-3p mimics
GSE59349 The differential miRNA expression induces M2 macrophage polarization and mediates their profibrogenic effects

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
GE_BrightCorner 14.727124
DarkCorner 2.3980706
A_23_P146146 7.037774
A_23_P42935 9.046928
A_23_P117082 13.997708
A_23_P2683 11.287287
A_24_P358131 6.3988576
A_33_P3367647 6.1748595
A_23_P157316 7.8552933
A_32_P14850 13.87304
A_23_P158596 9.238543
A_23_P350107 10.423829
A_23_P388190 11.257421
A_23_P106544 12.759
A_33_P3219745 3.7309806
A_32_P85539 9.591721
A_23_P94998 10.259682
A_33_P3235677 2.3347552
A_23_P417014 5.8022094
A_23_P103905 11.221956

Total number of rows: 34183

Table truncated, full table size 761 Kbytes.




Supplementary file Size Download File type/resource
GSM1694105_Con-Aso-2-1.txt.gz 2.0 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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