|
Status |
Public on Apr 20, 2007 |
Title |
HSC_Zfx-cko_Replicate1 |
Sample type |
RNA |
|
|
Source name |
Bone Marrow sorted Zfx-cko HSC (Lin-, Sca1+, c-Kit+)
|
Organism |
Mus musculus |
Characteristics |
5 x 10^4 Zfx-cko LSK (Lin-, Sca1+, c-Kit+) cells were sorted directly into Trizol Reagent (Invitrogen) form pooled bone marrow cells from Zfx-cko mice.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated and quantified using the Quant-IT RNA Assay Kit (Invitrogen)
|
Label |
Biotin
|
Label protocol |
Ambion MessageAmp II aRNA protocol Two-round amplification and labeling
|
|
|
Hybridization protocol |
Hybridization performed on a Affymetrix FS450/250 Fluidics Station using the FS450_0002 script.
|
Scan protocol |
Array scanning and raw data processing were done using GCOS 1.4 software (Affymetrix)
|
Description |
Murine Zfx-cko HSC Replicate1
|
Data processing |
Array scanning and raw data processing were done using
GCOS 1.4 software (Affymetrix)
|
|
|
Submission date |
Feb 19, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Jose M Galan |
Organization name |
Columbia University Medical Center
|
Street address |
701 W 168th St Room 609
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7069 |
Zfx controls the self-renewal of embryonic and hematopoietic stem cells |
|
Relations |
Reanalyzed by |
GSE119085 |