|
| Status |
Public on Apr 20, 2007 |
| Title |
HSC_Zfx-flox_Replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone Marrow sorted Zfx-flox HSC (Lin-, Sca1+, c-Kit+)
|
| Organism |
Mus musculus |
| Characteristics |
5 x 10^4 Zfx-flox LSK (Lin-, Sca1+, c-Kit+) cells were sorted directly into Trizol Reagent (Invitrogen) form pooled bone marrow cells from Zfx-flox mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated and quantified using the Quant-IT RNA Assay Kit (Invitrogen)
|
| Label |
Biotin
|
| Label protocol |
Ambion MessageAmp II aRNA protocol
Two-round amplification and labeling
|
| |
|
| Hybridization protocol |
Hybridization performed on a Affymetrix FS450/250 Fluidics Station using the FS450_0002 script.
|
| Scan protocol |
Array scanning and raw data processing were done using
GCOS 1.4 software (Affymetrix)
|
| Description |
Murine Zfx-flox HSC Replicate1
|
| Data processing |
Array scanning and raw data processing were done using
GCOS 1.4 software (Affymetrix)
|
| |
|
| Submission date |
Feb 19, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jose M Galan |
| Organization name |
Columbia University Medical Center
|
| Street address |
701 W 168th St Room 609
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7069 |
Zfx controls the self-renewal of embryonic and hematopoietic stem cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
