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Sample GSM1697424 Query DataSets for GSM1697424
Status Public on Jun 15, 2016
Title 86376_M430-2_2_STOMACH_MGI_GENE-4
Sample type RNA
 
Source name Normal Diet_STOMACH
Organism Mus musculus
Characteristics group: Normal Diet
genotype: C57BL/6J
tissue: STOMACH
animal: 32
Growth protocol Nine-week-old male, ob/ob (B6.V-Lepob/J), and ob lean control (Lepob, heterozygote from the colony) mice were purchased from Jackson Lab (Bar Harbor, Main) and acclimatized to a constant temperature and humidity (72°F and 50% relative humidity with a 12 h light and dark cycle from 05:00 h to 17:00 h) and had free access to food (LabDiet 5K20, LabDiet, St. Louis, MO) and water. At the age of twelve weeks, all animals were fasted for four hours in the morning and euthanized at 1pm under isoflurane anathesia. Nine-week-old male, C57BL/6J mice were purchased from Jackson Lab (Bar Harbor, Main) and acclimatized to a constant temperature and humidity (72°F and 50% relative humidity with a 12 h light and dark cycle from 05:00 h to 17:00 h) for one week with free access to food (LabDiet 5001, LabDiet, St. Louis, MO) and water. After acclimation, half of the mice (n=6) were kept on the regular chow diet (LabDiet 5001) and half of them (n=6) were switched to TD93075 (Harlan Teklad, Madison, Wisconsin), a high-fat diet with 54.8%Kcal from fat for four weeks. At the end of the study, all animals were fasted for four hours in the morning and euthanized at 1pm under isoflurane anesthesia.
Extracted molecule total RNA
Extraction protocol Gastrointestinal tissues (whole stomach and about 1cm of intestinal samples from the middle of each part of the intestine) were collected. Finally a 1cm3 segment of the inferior right lobe of the liver was taken. Segments were immediately snap frozen in liquid nitrogen and stored at -80°C for subsequent RNA extraction. RNA extraction was performed using the RNeasy Mini kit (Qiagen Inc, Valencia, Calif). Total RNA yield and purity was estimated by UV spectroscopy (Nanodrop ND-1000 Spectrophotometer; Nanodrop Technologies, Wilmington, DE, USA) and RNA quality was assessed on an Agilent 2100 Bioanalyzer. Subsequently mRNA expression analysis was carried out.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol The biotinylated cRNA was prepared and hybridized to the Mouse 430A_2.0 GeneChip in accordance with Affymetrix protocols.
Scan protocol GeneChips were scanned by the Affymetrix GeneChip Scanner.
Description 86376_M430-2_2_STOMACH_MGI_GENE-4
Data processing Microarray data was preprocessed using RMA implemented in R with BrainArray custom CDF.
 
Submission date May 27, 2015
Last update date Jun 15, 2016
Contact name Jing Chen
Organization name CCHMC
Street address 3333 Burnet Ave
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL19559
Series (1)
GSE69306 Significant obesity associated gene expression changes are in the stomach but not intestines in obese mice

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
100008567_at 6.948212916
100009600_at 5.054818115
100012_at 3.531108274
100017_at 8.681738502
100019_at 7.878764381
100034251_at 6.667240979
100034739_at 4.8271983
100034748_at 4.380397844
100036520_at 4.181085887
100036521_at 8.138348625
100036523_at 4.858897155
100036768_at 4.715489422
100037258_at 9.199257095
100037262_at 4.678869506
100037278_at 4.596856381
100038347_at 9.883809407
100038358_at 4.109117911
100038371_at 3.770941084
100038392_at 4.473749915
100038398_at 4.228728891

Total number of rows: 17607

Table truncated, full table size 366 Kbytes.




Supplementary file Size Download File type/resource
GSM1697424_EA09031_86376_M430-2_2_STOMACH_MGI_GENE-4.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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