|
| Status |
Public on May 29, 2015 |
| Title |
IL7_Del_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Setdb1 Δ/Δ pro-B cell, cultured in IL-7
|
| Organism |
Mus musculus |
| Characteristics |
cell type: pro-B cell
tissue: cultured in IL-7
bcl2 transgene: bcl-2 transgenic
setdb1: Deleted
|
| Growth protocol |
Murine bone marrow-derived pro-B cells were isolated and purified by FACS. Cells were then clutured for four days in media containing 5 ng/µl of IL-7 (Invitrogen, PMC0075).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using Qiagen Rneasy kits according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Purified RNA was amplified using Nugen Ovation kits and labeled using Nugen Encore Biotin Kit.
|
| |
|
| Hybridization protocol |
Products were hybridized to Affymetrix Mouse Gene 1.0 Array according to the manufacturer's instructions.
|
| Scan protocol |
GS3000
|
| Data processing |
Gene expression levels were determined using the Affymetrix Expression Console software (v1.2.0.20) with exon-level RMA analysis and default settings.
|
| |
|
| Submission date |
May 29, 2015 |
| Last update date |
May 29, 2015 |
| Contact name |
Patrick Collins |
| E-mail(s) |
patlennycollins@yahoo.com
|
| Organization name |
Washington University
|
| Department |
Pathology and Immunology
|
| Lab |
Oltz
|
| Street address |
1217 Oakleaf dr
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63119 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (2) |
| GSE69378 |
SETDB1 Represses Endogenous and Exogenous Retroviruses in B Lymphocytes [array] |
| GSE69504 |
SETDB1 Represses Endogenous and Exogenous Retroviruses in B Lymphocytes |
|
