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Sample GSM170283 Query DataSets for GSM170283
Status Public on Dec 11, 2007
Title Day 1 control 2
Sample type RNA
 
Source name rat lung, day1, ctrl
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day1, ctrl
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 139.900000 P 0.000127
AFFX-BioB-3_st 5.100000 A 0.891021
AFFX-BioB-5_at 110.500000 P 0.007620
AFFX-BioB-5_st 28.800000 A 0.089478
AFFX-BioB-M_at 228.900000 P 0.000127
AFFX-BioB-M_st 21.400000 A 0.354453
AFFX-BioC-3_at 294.700000 P 0.000095
AFFX-BioC-3_st 2.200000 A 0.814869
AFFX-BioC-5_at 439.000000 P 0.000127
AFFX-BioC-5_st 4.900000 A 0.860518
AFFX-BioDn-3_at 1321.200000 P 0.000147
AFFX-BioDn-3_st 25.200000 A 0.165861
AFFX-BioDn-5_at 297.000000 P 0.000127
AFFX-BioDn-5_st 39.500000 A 0.185131
AFFX-CreX-3_at 5981.800000 P 0.000044
AFFX-CreX-3_st 5.500000 A 0.425962
AFFX-CreX-5_at 2753.300000 P 0.000044
AFFX-CreX-5_st 18.900000 A 0.116113
AFFX-DapX-3_at 2.100000 A 0.876428
AFFX-DapX-5_at 4.800000 A 0.411380

Total number of rows: 8799

Table truncated, full table size 296 Kbytes.




Supplementary file Size Download File type/resource
GSM170283.cel.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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