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Status
Public on Dec 11, 2007
Title
Day 21 smoke exposure 2
Sample type
RNA
Source name
rat lung, day21, smoke
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day21, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
89.100000
P
0.000095
AFFX-BioB-3_st
3.200000
A
0.804734
AFFX-BioB-5_at
89.600000
P
0.003595
AFFX-BioB-5_st
27.400000
A
0.083592
AFFX-BioB-M_at
91.100000
P
0.000070
AFFX-BioB-M_st
21.100000
A
0.147939
AFFX-BioC-3_at
166.300000
P
0.000060
AFFX-BioC-3_st
1.300000
A
0.794268
AFFX-BioC-5_at
237.500000
P
0.000095
AFFX-BioC-5_st
3.200000
A
0.883887
AFFX-BioDn-3_at
1487.200000
P
0.000095
AFFX-BioDn-3_st
18.200000
A
0.165861
AFFX-BioDn-5_at
331.200000
P
0.000081
AFFX-BioDn-5_st
35.800000
A
0.089478
AFFX-CreX-3_at
4687.300000
P
0.000044
AFFX-CreX-3_st
2.200000
A
0.686277
AFFX-CreX-5_at
2164.200000
P
0.000044
AFFX-CreX-5_st
8.000000
A
0.250796
AFFX-DapX-3_at
1.400000
A
0.712257
AFFX-DapX-5_at
0.300000
A
0.574038
8799 295 Kbytes .Supplementary file Size Download File type/resource GSM170303.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
