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Status
Public on Dec 11, 2007
Title
Day 28 smoke exposure 1
Sample type
RNA
Source name
rat lung, day28, smoke
Organism
Rattus norvegicus
Characteristics
Strain : SD Gender : Male Tissue : Lung
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day28, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85
Series (1)
GSE7079
Chronic rat exposure to cigarette smoke
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-3_at
91.600000
P
0.000060
AFFX-BioB-3_st
4.900000
A
0.794268
AFFX-BioB-5_at
62.200000
P
0.013811
AFFX-BioB-5_st
27.900000
A
0.067678
AFFX-BioB-M_at
151.700000
P
0.000297
AFFX-BioB-M_st
18.500000
A
0.327079
AFFX-BioC-3_at
189.400000
P
0.000070
AFFX-BioC-3_st
1.200000
A
0.937071
AFFX-BioC-5_at
297.600000
P
0.000127
AFFX-BioC-5_st
1.800000
A
0.949771
AFFX-BioDn-3_at
1381.400000
P
0.000147
AFFX-BioDn-3_st
22.600000
A
0.067678
AFFX-BioDn-5_at
249.500000
P
0.000297
AFFX-BioDn-5_st
30.000000
A
0.175328
AFFX-CreX-3_at
4089.600000
P
0.000044
AFFX-CreX-3_st
5.300000
A
0.455413
AFFX-CreX-5_at
2185.900000
P
0.000044
AFFX-CreX-5_st
14.400000
A
0.216524
AFFX-DapX-3_at
1.000000
A
0.927300
AFFX-DapX-5_at
4.800000
A
0.205732
Total number of rows: 8799 Table truncated, full table size 295 Kbytes .Supplementary file
Size
Download
File type/resource
GSM170308.cel.gz
1.9 Mb
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CEL
Processed data included within Sample table