NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM170308 Query DataSets for GSM170308
Status Public on Dec 11, 2007
Title Day 28 smoke exposure 1
Sample type RNA
 
Source name rat lung, day28, smoke
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day28, smoke
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 91.600000 P 0.000060
AFFX-BioB-3_st 4.900000 A 0.794268
AFFX-BioB-5_at 62.200000 P 0.013811
AFFX-BioB-5_st 27.900000 A 0.067678
AFFX-BioB-M_at 151.700000 P 0.000297
AFFX-BioB-M_st 18.500000 A 0.327079
AFFX-BioC-3_at 189.400000 P 0.000070
AFFX-BioC-3_st 1.200000 A 0.937071
AFFX-BioC-5_at 297.600000 P 0.000127
AFFX-BioC-5_st 1.800000 A 0.949771
AFFX-BioDn-3_at 1381.400000 P 0.000147
AFFX-BioDn-3_st 22.600000 A 0.067678
AFFX-BioDn-5_at 249.500000 P 0.000297
AFFX-BioDn-5_st 30.000000 A 0.175328
AFFX-CreX-3_at 4089.600000 P 0.000044
AFFX-CreX-3_st 5.300000 A 0.455413
AFFX-CreX-5_at 2185.900000 P 0.000044
AFFX-CreX-5_st 14.400000 A 0.216524
AFFX-DapX-3_at 1.000000 A 0.927300
AFFX-DapX-5_at 4.800000 A 0.205732

Total number of rows: 8799

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM170308.cel.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap