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Sample GSM170312 Query DataSets for GSM170312
Status Public on Dec 11, 2007
Title Day 1 control 5
Sample type RNA
 
Source name rat lung, day1, ctrl
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day1, ctrl
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 108.000000 P 0.000754
AFFX-BioB-3_st 8.400000 A 0.891021
AFFX-BioB-5_at 68.500000 M 0.050229
AFFX-BioB-5_st 40.500000 A 0.139482
AFFX-BioB-M_at 123.100000 P 0.001248
AFFX-BioB-M_st 25.900000 A 0.425962
AFFX-BioC-3_at 176.900000 P 0.000070
AFFX-BioC-3_st 2.300000 A 0.904333
AFFX-BioC-5_at 301.200000 P 0.000147
AFFX-BioC-5_st 3.900000 A 0.937071
AFFX-BioDn-3_at 1332.400000 P 0.000127
AFFX-BioDn-3_st 33.300000 A 0.175328
AFFX-BioDn-5_at 219.400000 P 0.000225
AFFX-BioDn-5_st 26.400000 A 0.287743
AFFX-CreX-3_at 4330.400000 P 0.000044
AFFX-CreX-3_st 5.600000 A 0.514890
AFFX-CreX-5_at 2144.400000 P 0.000044
AFFX-CreX-5_st 24.800000 A 0.123572
AFFX-DapX-3_at 2.900000 A 0.804734
AFFX-DapX-5_at 9.900000 A 0.185131

Total number of rows: 8799

Table truncated, full table size 296 Kbytes.




Supplementary file Size Download File type/resource
GSM170312.cel.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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