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Status
Public on Dec 11, 2007
Title
Day 1 control 5
Sample type
RNA
Source name
rat lung, day1, ctrl
Organism
Rattus norvegicus
Characteristics
Strain : SD Gender : Male Tissue : Lung
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day1, ctrl
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85
Series (1)
GSE7079
Chronic rat exposure to cigarette smoke
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-3_at
108.000000
P
0.000754
AFFX-BioB-3_st
8.400000
A
0.891021
AFFX-BioB-5_at
68.500000
M
0.050229
AFFX-BioB-5_st
40.500000
A
0.139482
AFFX-BioB-M_at
123.100000
P
0.001248
AFFX-BioB-M_st
25.900000
A
0.425962
AFFX-BioC-3_at
176.900000
P
0.000070
AFFX-BioC-3_st
2.300000
A
0.904333
AFFX-BioC-5_at
301.200000
P
0.000147
AFFX-BioC-5_st
3.900000
A
0.937071
AFFX-BioDn-3_at
1332.400000
P
0.000127
AFFX-BioDn-3_st
33.300000
A
0.175328
AFFX-BioDn-5_at
219.400000
P
0.000225
AFFX-BioDn-5_st
26.400000
A
0.287743
AFFX-CreX-3_at
4330.400000
P
0.000044
AFFX-CreX-3_st
5.600000
A
0.514890
AFFX-CreX-5_at
2144.400000
P
0.000044
AFFX-CreX-5_st
24.800000
A
0.123572
AFFX-DapX-3_at
2.900000
A
0.804734
AFFX-DapX-5_at
9.900000
A
0.185131
Total number of rows: 8799 Table truncated, full table size 296 Kbytes .Supplementary file
Size
Download
File type/resource
GSM170312.cel.gz
1.9 Mb
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CEL
Processed data included within Sample table