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Status
Public on Dec 11, 2007
Title
Day 3 smoke exposure 6
Sample type
RNA
Source name
rat lung, day3, smoke
Organism
Rattus norvegicus
Characteristics
Strain : SD Gender : Male Tissue : Lung
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day3, smoke
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85
Series (1)
GSE7079
Chronic rat exposure to cigarette smoke
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
AFFX-BioB-3_at
146.900000
P
0.000147
AFFX-BioB-3_st
7.000000
A
0.868639
AFFX-BioB-5_at
97.400000
P
0.021902
AFFX-BioB-5_st
36.000000
A
0.116113
AFFX-BioB-M_at
162.800000
P
0.000509
AFFX-BioB-M_st
22.800000
A
0.340661
AFFX-BioC-3_at
283.200000
P
0.000044
AFFX-BioC-3_st
2.900000
A
0.883887
AFFX-BioC-5_at
404.800000
P
0.000127
AFFX-BioC-5_st
3.800000
A
0.897835
AFFX-BioDn-3_at
2028.100000
P
0.000070
AFFX-BioDn-3_st
36.000000
A
0.185131
AFFX-BioDn-5_at
323.500000
P
0.000095
AFFX-BioDn-5_st
25.300000
A
0.313723
AFFX-CreX-3_at
6128.100000
P
0.000044
AFFX-CreX-3_st
4.300000
A
0.470241
AFFX-CreX-5_at
2994.900000
P
0.000044
AFFX-CreX-5_st
20.600000
A
0.195266
AFFX-DapX-3_at
1.700000
A
0.969024
AFFX-DapX-5_at
2.400000
A
0.588620
Total number of rows: 8799 Table truncated, full table size 296 Kbytes .Supplementary file
Size
Download
File type/resource
GSM170322.cel.gz
1.8 Mb
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CEL
Processed data included within Sample table