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Sample GSM170322 Query DataSets for GSM170322
Status Public on Dec 11, 2007
Title Day 3 smoke exposure 6
Sample type RNA
 
Source name rat lung, day3, smoke
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day3, smoke
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 146.900000 P 0.000147
AFFX-BioB-3_st 7.000000 A 0.868639
AFFX-BioB-5_at 97.400000 P 0.021902
AFFX-BioB-5_st 36.000000 A 0.116113
AFFX-BioB-M_at 162.800000 P 0.000509
AFFX-BioB-M_st 22.800000 A 0.340661
AFFX-BioC-3_at 283.200000 P 0.000044
AFFX-BioC-3_st 2.900000 A 0.883887
AFFX-BioC-5_at 404.800000 P 0.000127
AFFX-BioC-5_st 3.800000 A 0.897835
AFFX-BioDn-3_at 2028.100000 P 0.000070
AFFX-BioDn-3_st 36.000000 A 0.185131
AFFX-BioDn-5_at 323.500000 P 0.000095
AFFX-BioDn-5_st 25.300000 A 0.313723
AFFX-CreX-3_at 6128.100000 P 0.000044
AFFX-CreX-3_st 4.300000 A 0.470241
AFFX-CreX-5_at 2994.900000 P 0.000044
AFFX-CreX-5_st 20.600000 A 0.195266
AFFX-DapX-3_at 1.700000 A 0.969024
AFFX-DapX-5_at 2.400000 A 0.588620

Total number of rows: 8799

Table truncated, full table size 296 Kbytes.




Supplementary file Size Download File type/resource
GSM170322.cel.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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