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Status
Public on Dec 11, 2007
Title
Day 3 control 8
Sample type
RNA
Source name
rat lung, day3, ctrl
Organism
Rattus norvegicus Characteristics
Strain : SD
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
Hybridization protocol
Standard Affymetrix protocol
Scan protocol
Standard Affymetrix protocol
Description
rat lung, day3, ctrl
Data processing
The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
Submission date
Feb 20, 2007
Last update date
Dec 11, 2007
Contact name
Christopher S Stevenson
Organization name
Novartis Institutes for BioMedical Research
Department
Respiratory Diseases
Street address
Wimblehurst Road
City
Horsham
ZIP/Postal code
RH12 5AB
Country
United Kingdom
Platform ID
GPL85 Series (1)
GSE7079 Chronic rat exposure to cigarette smoke
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-3_at
145.000000
P
0.000070
AFFX-BioB-3_st
10.600000
A
0.737173
AFFX-BioB-5_at
112.100000
P
0.008440
AFFX-BioB-5_st
39.200000
A
0.083592
AFFX-BioB-M_at
303.700000
P
0.000110
AFFX-BioB-M_st
30.600000
A
0.262827
AFFX-BioC-3_at
404.100000
P
0.000070
AFFX-BioC-3_st
3.500000
A
0.804734
AFFX-BioC-5_at
497.000000
P
0.000110
AFFX-BioC-5_st
4.100000
A
0.910522
AFFX-BioDn-3_at
2048.600000
P
0.000081
AFFX-BioDn-3_st
32.300000
A
0.239063
AFFX-BioDn-5_at
398.400000
P
0.000081
AFFX-BioDn-5_st
35.500000
A
0.275146
AFFX-CreX-3_at
6412.000000
P
0.000044
AFFX-CreX-3_st
25.700000
A
0.411380
AFFX-CreX-5_at
3333.200000
P
0.000044
AFFX-CreX-5_st
12.900000
A
0.239063
AFFX-DapX-3_at
5.200000
A
0.814869
AFFX-DapX-5_at
15.300000
A
0.116113
8799 296 Kbytes .Supplementary file Size Download File type/resource GSM170346.cel.gz
1.9 Mb
(ftp) (http) CEL
Processed data included within Sample table
