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Sample GSM170349 Query DataSets for GSM170349
Status Public on Dec 11, 2007
Title Day 5 control 7
Sample type RNA
 
Source name rat lung, day5, ctrl
Organism Rattus norvegicus
Characteristics Strain : SD
Gender : Male
Tissue : Lung
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturers instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description rat lung, day5, ctrl
Data processing The data was analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling of as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Feb 20, 2007
Last update date Dec 11, 2007
Contact name Christopher S Stevenson
Organization name Novartis Institutes for BioMedical Research
Department Respiratory Diseases
Street address Wimblehurst Road
City Horsham
ZIP/Postal code RH12 5AB
Country United Kingdom
 
Platform ID GPL85
Series (1)
GSE7079 Chronic rat exposure to cigarette smoke

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 94.800000 P 0.000662
AFFX-BioB-3_st 4.500000 A 0.945787
AFFX-BioB-5_at 57.200000 P 0.030976
AFFX-BioB-5_st 45.400000 A 0.062929
AFFX-BioB-M_at 148.300000 P 0.000662
AFFX-BioB-M_st 25.400000 A 0.340661
AFFX-BioC-3_at 270.500000 P 0.000110
AFFX-BioC-3_st 3.000000 A 0.760937
AFFX-BioC-5_at 267.600000 P 0.000258
AFFX-BioC-5_st 5.200000 A 0.897835
AFFX-BioDn-3_at 1389.600000 P 0.000195
AFFX-BioDn-3_st 18.500000 A 0.368438
AFFX-BioDn-5_at 263.100000 P 0.000195
AFFX-BioDn-5_st 27.400000 A 0.227636
AFFX-CreX-3_at 4139.400000 P 0.000044
AFFX-CreX-3_st 3.000000 A 0.686277
AFFX-CreX-5_at 1937.300000 P 0.000044
AFFX-CreX-5_st 20.300000 A 0.313723
AFFX-DapX-3_at 1.200000 A 0.957038
AFFX-DapX-5_at 9.200000 A 0.354453

Total number of rows: 8799

Table truncated, full table size 296 Kbytes.




Supplementary file Size Download File type/resource
GSM170349.cel.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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